Mouse Interferon alpha,IFN-α ELISA Kit

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Mouse Interferon alpha,IFN-α ELISA Kit

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Protocol

Code	CSB-E08639m
Size	96T,5×96T,10×96T
Price	Request a Quote
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Target Name

IFN-α

Alternative Names

N/A

Abbreviation

IFN-α

Uniprot No.

N A

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6pg/mL-1000pg/mL

Sensitivity

3.9pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-105
1:2	Average %	94
	Range %	87-101
1:4	Average %	97
	Range %	90-103
1:8	Average %	93
	Range %	87-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	3.015	3.234	3.125	2.956
500	2.580	2.467	2.524	2.355
250	1.831	1.878	1.855	1.686
125	1.079	1.129	1.104	0.935
62.5	0.770	0.721	0.745	0.577
31.2	0.437	0.453	0.445	0.276
15.6	0.337	0.324	0.330	0.161
0	0.173	0.165	0.169

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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