Mouse Interferon epsilon(IFNE) ELISA kit

Code CSB-EL011049MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
interferon, epsilon
Alternative Names
Ifne ELISA Kit; Ifne1 ELISA Kit; Ifnt1Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit; Interferon tau-1 ELISA Kit
Abbreviation
IFNE
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 83-88
1:2 Average % 94
Range % 89-98
1:4 Average % 103
Range % 97-108
1:8 Average % 99
Range % 95-104
Recovery
The recovery of mouse IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-101
EDTA plasma (n=4) 89 83-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.271 2.366 2.319 2.143
500 1.563 1.625 1.594 1.418
250 0.944 0.956 0.950 0.774
125 0.582 0.581 0.582 0.406
62.5 0.382 0.377 0.380 0.204
31.2 0.274 0.289 0.282 0.106
15.6 0.220 0.235 0.228 0.052
0 0.172 0.179 0.176  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse IFNE ELISA Kit was designed for the quantitative measurement of Mouse IFNE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral, including HSV-2, and bacterial, including Chlamydia muridarum, genital infections.
Gene References into Functions
  1. IFN-epsilon is constitutively expressed by cells of the reproductive tract and is inefficiently secreted by fibroblasts and cell lines. PMID: 23951133
  2. IFN-epsilon could promote migration of antigen-specific CD8(+)T cells to the gut. PMID: 22617838
  3. Ifn-epsilon-deficient mice had increased susceptibility to infection of the female reproductive tract by the common sexually transmitted infections (STIs) herpes simplex virus 2 and Chlamydia muridarum. PMID: 23449591
  4. interactions between estradiol and IFN-tau in the context of the overall immune response during autoimmune orchitis PMID: 21787220
Subcellular Location
Secreted.
Protein Families
Alpha/beta interferon family
Tissue Specificity
Expressed at very high levels in uterus and, at much lower levels, in ovary and cervix. Very low levels, if any, in other organs. In the endometrium, expressed in the luminal and glandular epithelial cells (at protein level).
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