Mouse Interleukin-17F(IL17F) ELISA kit

Instructions
Code CSB-EL011601MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name interleukin 17F
Alternative Names Il17fInterleukin-17F ELISA Kit; IL-17F ELISA Kit
Abbreviation IL17F
Uniprot No. Q7TNI7
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 91-103
1:2 Average % 91
Range % 83-95
1:4 Average % 94
Range % 86-98
1:8 Average % 95
Range % 90-100
Recovery
The recovery of mouse IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 94-101
EDTA plasma (n=4) 101 90-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 1.796 1.829 1.813 1.597
100 1.346 1.368 1.357 1.141
50 0.930 0.976 0.953 0.737
25 0.712 0.734 0.723 0.507
12.5 0.515 0.527 0.521 0.305
6.25 0.407 0.423 0.415 0.199
3.12 0.311 0.319 0.315 0.099
0 0.218 0.214 0.216  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Ligand for IL17RA and IL17RC
Gene References into Functions
  1. our results indicate that Th17 cells contribute to the airway vascular remodeling in asthma by mediating EPC chemotaxis, as well as PMVEC tube formation, via IL-17A rather than IL-17F. PMID: 25780043
  2. Data indicate that IL-17A repressed IL-17F secretion in vitro and in vivo. PMID: 24600029
  3. The concentrations in plasma of IL-17A, IL-17F, and the IL-17AF heterodimer all were increased greatly in mice after endotoxemia. PMID: 23499051
  4. IL-17A and IL-17F secreted by CD4+Th17 cells specific to lung self-antigens are critical mediators of autoimmunity leading to the pathogenesis of obliterative airway disease. PMID: 23325004
  5. The Th17 subset of T-lymphocytes produces IL-17F to stimulate bone healing. PMID: 22768215
  6. IL-17F is not required for development of T helper cell (Th)2-type allergic airway inflammation. PMID: 22942422
  7. Together, our findings demonstrate a protective role for IL-17F in colon cancer development, possibly via inhibiting tumor angiogenesis PMID: 22509371
  8. These results demonstrate that IL-17A/F plays a critical role in Legionella pneumophila pneumonia, probably through induction of proinflammatory cytokines and accumulation of neutrophils at the infection site. PMID: 22144493
  9. CNS2 is sufficient and necessary for Il17 and optimal Il17f gene transcription in Th17 cells PMID: 22244845
  10. IL-17F was produced in peritoneal macrophages after TLR4 activation, peaking after 12 h. This effect was completely dependent on the presence of MyD88. The copresence of the complement activation product C5a amplified IL-17F production. PMID: 21859896
  11. This work demonstrates the important roles of both IL-17 signaling and neutrophils in clearing this pathogen and surviving pulmonary Bacillus anthracis infection. PMID: 22025514
  12. these results suggest the proinflammatory and essential role of IL-17F to develop spontaneous intestinal tumorigenesis in Apc(Min/+) mice in the presence of IL-17A. PMID: 21939640
  13. Data show that Mel-18 and Ezh2 positively regulate the expression of Il17a and Il17f. PMID: 21674483
  14. Increased resistance to infection in a mouse model of tularemia was associated with a specific increase in IL-17A/F PMID: 20176744
  15. IL-17A and IL-17F induce the production of chemokines MCP-1 and MIP-2 via MAPK pathways (p38 MAPK and ERK1/2), as well as mRNA transcription and protein translation and have synergistic effects with TNF-alpha and IL-1beta in cultured mesangial cells. PMID: 20042461
  16. Intranasal administration of adenovirus expressing IL-17F results in bronchoalveolar lavage neutrophilia and inflammatory gene expression in the lung. PMID: 12077275
  17. a role for IL-17F in the induction of neutrophilia in the lungs and in the exacerbation of Ag-induced pulmonary inflammation. PMID: 15477493
  18. THi cell differentiation is associated with epigenetic changes in the IL-17-IL-17F locus, which suggests novel mechanisms in T cell functional regulation. PMID: 17218320
  19. study found that THi cells upon stimulation produce not only IL-17 and IL-17F homodimers but also the IL-17A/F heterodimer; IL-17A/F cytokine represents another mechanism whereby T cells regulate inflammatory responses PMID: 17452998
  20. In sham-challenged mice, IL-17F was not expressed in the lungs, while, in contrast, IL-17F was predominantly expressed in bronchial epithelial cells in addition to the infiltrating inflammatory cells in OVA/OVA mice. IL-17F mayplay a role in asthma. PMID: 17541285
  21. IL-17F is an important regulator of inflammatory responses that seems to function differently than IL-17 in immune responses and diseases. PMID: 18411338
  22. IL-17F/IL-17R interaction stimulates granulopoiesis in mice. PMID: 18723265
  23. IL-17F may be involved in psoriasis via, in part, the activation of ERK1/2 and the induction of IL-8 in keratinocytes PMID: 18830271
  24. RORgamma controls IL-17A and IL-17F production, and these cytokines have a redundant but highly pathogenic role in gut inflammation. PMID: 18992745
  25. Both IL-17A and IL-17F can signal via the IL-17RA; however, IL-17F does so at a 1- to 2-log higher concentration than IL-17A. PMID: 19017936
  26. Data suggest that both IL-17A and IL-17F, while prominently expressed by an encephalitogenic T cell population, may only marginally contribute to the development of autoimmune CNS disease. PMID: 19075395
  27. IL-17F and IL-17A were involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium. IL-17A was produced mainly in T cells, whereas IL-17F was produced in T cells, innate immune cells, and epithelial cells PMID: 19144317
  28. IL-17A mediated suppression of IL-17F production and secretion requires IL-17RA and is relevant to maintain the normal set point of blood neutrophil counts in vivo PMID: 19542376
  29. T cells lacking Itk exhibit decreased interleukin-17A expression in vitro and in vivo, despite relatively normal expression of retinoic acid receptor-related orphan receptor-gammaT and IL-17F. PMID: 19818650

Show More

Hide All

Subcellular Location Secreted
Protein Families IL-17 family
Tissue Specificity Expressed by a subset of activated T-cells in the lamina propria.
Database Links

KEGG: mmu:257630

STRING: 10090.ENSMUSP00000046960

UniGene: Mm.222807

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1