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| Code | CSB-E13046m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:400 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 85-105 | |||||
| The recovery of mouse KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 100 | 95-103 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This mouse FGF7 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse FGF1 in multiple samples, including serum, plasma, cell culture supernates, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the FGF7 levels in the sample and is measured at 450 nm through a microplate reader.
FGF7 is a mesenchyme-specific heparin-binding growth factor that stimulates cell proliferation, differentiation, migration, and vascular angiogenesis. Mesenchymal-derived FGF7 binds receptors on epithelial to mediate epithelial-mesenchymal interactions in various organs, including the reproductive tract. FGF7 exerts its effect in a paracrine manner and specifically activates the receptor tyrosine kinase FGFR2IIIb. FGF7 binding promotes FGFR2 dimerization, eliciting several signal transduction pathways, including RAS-MAPK and PI3K-Akt signaling pathways, which play fundamental roles in tumor progression.
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