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Mouse Mannan-binding lectin serine protease 2(MASP2) ELISA kit

Citations (1) Protocol
Code CSB-EL013510MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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Product Details

Target Name
mannan-binding lectin serine peptidase 2
Alternative Names
Masp2Mannan-binding lectin serine protease 2 ELISA Kit; EC 3.4.21.104 ELISA Kit; MBL-associated serine protease 2 ELISA Kit; Mannose-binding protein-associated serine protease 2 ELISA Kit; MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain; Mannan-binding lectin serine protease 2 B chain] ELISA Kit
Abbreviation
MASP2
Uniprot No.
Q91WP0
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
125 pg/mL-8000 pg/mL
Sensitivity
31.2 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MASP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:100 Average % 92    
Range % 89-95    
1:200 Average % 101    
Range % 97-106    
1:400 Average % 86    
Range % 83-90    
1:800 Average % 102    
Range % 97-107    
Recovery
The recovery of mouse MASP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 85-93    
EDTA plasma (n=4) 100 95-105    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average      
8000 0.100 0.106 0.103      
4000 0.243 0.240 0.242      
2000 0.358 0.361 0.360      
1000 0.516 0.531 0.524      
500 0.701 0.740 0.721      
250 1.027 1.100 1.064      
125 1.507 1.496 1.502      
0 2.311 2.298 2.305      
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse MASP2 ELISA Kit was designed for the quantitative measurement of Mouse MASP2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.2 pg/mL.

Citations

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Target Background

Function
(From Uniprot)
Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.
Gene References into Functions
  1. MASP-2 has an essential role in the mediation of cerebral ischemia-reperfusion injury. PMID: 27577570
  2. this study shows that MASP-2 contributes to the development of collagen Ab-induced arthritis PMID: 28739878
  3. We induced anti-myeloperoxidase vasculitis in mice and showed that neither MASP-2- nor properdin-deficient mice were protected, suggesting that alternative pathway activation does not require properdin or the lectin pathway. PMID: 27235854
  4. Mannan-binding lectin-associated serine protease 2 is critical for the development of renal ischemia reperfusion injury and mediates tissue injury in the absence of complement C4. PMID: 24868011
  5. Gene targeting of mouse TDP-43 negatively affects Masp2 expression. PMID: 24740308
  6. ALI in H5N1-infected mice was caused by excessive complement activation, as demonstrated by deposition of C3, C5b-9, and MBL-C in lung tissue, and by up-regulation of MBL-associated serine protease-2 and the complement receptors C3aR and C5aR. PMID: 23526211
  7. study corroborates the essential function of MASP-2 in the lectin pathway and highlights the importance of mannan-binding lectin-independent lectin pathway activation in the host defense against pneumococci PMID: 22792067
  8. Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury. PMID: 21502512
  9. Highly conserved intron sequences. Restricted expression contrasts with the ubiquitous expression of neighboring genes. PMID: 15672593
  10. Recombinant protein of masp2 was made and purified. PMID: 15826378
  11. MASP-2 is essential for the activation of C4 and sMAP plays a regulatory role in the activation of the lectin pathway. PMID: 17142762

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Subcellular Location
Secreted.
Protein Families
Peptidase S1 family
Tissue Specificity
Plasma.
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