Mouse Mast Cell Tryptase(MCT) ELISA Kit

Code CSB-E14326m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
Mast Cell Tryptase(MCT)
Alternative Names
Tpsab1 ELISA Kit; Mcpt7 ELISA Kit; Tryptase ELISA Kit; EC ELISA Kit; Mast cell protease 7 ELISA Kit; mMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
7.8 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %89-96
1:2Average %96
Range %91-102
1:4Average %97
Range %93-100
1:8Average %93
Range %88-99
The recovery of mouse MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9693-101
EDTA plasma (n=4)9184-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
20002.562 2.756 2.659 2.497
10002.173 2.213 2.193 2.031
5001.426 1.485 1.456 1.294
2500.801 0.845 0.823 0.661
1250.545 0.590 0.568 0.406
62.50.309 0.293 0.301 0.139
31.250.246 0.234 0.240 0.078
00.168 0.155 0.162  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Mouse MCT ELISA Kit was designed for the quantitative measurement of Mouse MCT protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

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Target Background

(From Uniprot)
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
Gene References into Functions
  1. transcriptional activation of mouse mast cell Protease-7 by activin and transforming growth factor-beta is inhibited by microphthalmia-associated transcription factor PMID: 14527958
  2. Because mMCP-6 and mMCP-7 can compensate for each other in this mouse disease model, the elimination of both tryptases is necessary to reveal the prominent roles of these serine proteases in joint inflammation and destruction. PMID: 18668540
  3. TGF-beta stimulates mmcp-7 transcription through the Smad3-Smad4 pathway as well as c-fos induction PMID: 16730810
Protein Families
Peptidase S1 family, Tryptase subfamily
Database Links
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