Mouse Melanoma-derived growth regulatory protein(MIA) ELISA kit

Code CSB-EL013798MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
melanoma inhibitory activity
Alternative Names
Mia ELISA Kit; Cdrap ELISA Kit; Mia1 ELISA Kit; Melanoma-derived growth regulatory protein ELISA Kit; Cartilage-derived retinoic acid-sensitive protein ELISA Kit; CD-RAP ELISA Kit; Melanoma inhibitory activity protein ELISA Kit
Abbreviation
MIA
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.7 pg/mL-300 pg/mL
Sensitivity
1.17 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %82-99
1:200Average %101
Range %97-105
1:400Average %92
Range %88-96
1:800Average %87
Range %85-89
Recovery
The recovery of mouse MIA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 104101-107
EDTA plasma (n=4)9692-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
3002.502 2.563 2.533 2.426
1502.256 2.212 2.234 2.127
751.698 1.680 1.689 1.582
37.50.859 0.899 0.879 0.772
18.750.514 0.536 0.525 0.418
9.40.301 0.326 0.314 0.207
4.70.192 0.172 0.182 0.075
00.107 0.106 0.107
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse MIA ELISA Kit was designed for the quantitative measurement of Mouse MIA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

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Target Background

Function
(From Uniprot)
Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Gene References into Functions
  1. The effects of MIA/CD-RAP on transcriptional regulation in chondrocytes, through the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. PMID: 24349210
  2. Attenuated LM expressing MIA. PMID: 22886976
  3. as observed in other knockout models of molecules important for cartilage development and differentiation, viability and functional integrity is reached by remarkable molecular redundancy in MIA/CD-RAP knockout mice. PMID: 24269712
  4. The cartilage protein melanoma inhibitory activity contributes to inflammatory arthritis. PMID: 24287514
  5. Expression analysis of cartilage tissue derived from MIA-/- mice revealed strong downregulation of nuclear RNA-binding protein 54-kDa. PMID: 21368873
  6. MIA/CD-RAP stabilizes cartilage differentiation and inhibits differentiation into bone potentially by regulating signaling processes during differentiation. PMID: 20164682
  7. transcriptional mechanism by which CD-RAP expression is suppressed by IL-1 beta PMID: 12072435
  8. Cdrap/Mia is located between two housekeeping genes PMID: 15028289
  9. DNA promoter segment from -2,251 bp to -2,068 bp of the CD-RAP gene contains elements critical for gene expression. It demonstrates activation or repression of gene expression in vitro and in vivo based on cell type and content of transcription factors. PMID: 16250001
  10. A potential tumor suppressor role of Mia/Cd-rap in pituitary cells. PMID: 17914116
  11. Microarray analysis showed significant reductions in the expression of several proteins in lung misexpressing MIA. PMID: 18342301

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Subcellular Location
Secreted.
Protein Families
MIA/OTOR family
Tissue Specificity
All malignant melanoma cell lines tested and infrequently in glioma cell lines.
Database Links

KEGG: mmu:12587

UniGene: Mm.262447

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