Mouse Metallothionein-2(MT-2) ELISA Kit

Product Details

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Target Background

Product Details

Target Name

Metallothionein-2(MT-2)

Alternative Names

Mt2 ELISA Kit; Metallothionein-2 ELISA Kit; MT-2 ELISA Kit; Metallothionein-II ELISA Kit; MT-II ELISA Kit

Abbreviation

MT-2

Uniprot No.

P02798

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MT-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	102
	Range %	99-105
1:4	Average %	89
	Range %	86-93
1:8	Average %	94
	Range %	92-97

Recovery

The recovery of mouse MT-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	101-106
EDTA plasma (n=4)	95	92-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.975	2.022	1.999	1.842
500	1.362	1.377	1.370	1.213
250	0.811	0.862	0.837	0.680
125	0.433	0.439	0.436	0.279
62.5	0.304	0.330	0.317	0.160
31.2	0.262	0.274	0.268	0.111
15.6	0.214	0.220	0.217	0.060
0	0.163	0.150	0.157

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse MT-2 ELISA Kit was designed for the quantitative measurement of Mouse MT-2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Background

Function
(From Uniprot)

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Gene References into Functions

these results demonstrate that MT2 plays a role in CD4+ T cell activation by transducing reactive oxygen species signals into an increased [Zn2+]i that subsequently affects downstream effector function PMID: 27251638
Metallothioneins may have a protective role against post-burn inflammation and inflammatory organ damage, at least partly through inhibiting the p38 MAPK signaling. PMID: 26833479
The MT2 receptor activates Akt/GSK-3beta/CRMP-2 signaling and is necessary and sufficient to mediate functional axonogenesis and synaptic formation in central neurons. PMID: 25501601
Zn deficiency in utero induces fetal epigenetic alterations of Mt2 gene and these changes are being stored as an epigenetic memory until adulthood. PMID: 22917840
Knockdown of metallothionein 2 does not affect atrophy or oxidant activity in skeletal myotubes subject to serum reduction. PMID: 20798270
MT-null phenotype precludes lead-induced inclusion body formation and increases lead toxicity at the organ and cellular level despite reducing lead accumulation PMID: 11891201
MT-2 deficiency increases brain pathology in transgenic mice with astrocyte-targeted expression of interleukin 6. PMID: 11950277
In mouse spinal cord injury lead the up-regulation MT-2 expression at 1 day and MT-2 expression was observed in activated macrophages/microglia at 1 day. PMID: 15592854
MT2a has a role in oxidative stress, and the formation of MT disulfide bonds is involved in the regulation of zinc homeostasis PMID: 16243847
Mt-2 synthesis was induced by cilostazol. PMID: 17409522
Metallothionein II deficient mice exhibit impaired rate of maze learning but retained the information as well as the wild-type mice. The absence of functional MT-II does not result in significantly worse injury following 45 min of hypoxia-ischemia. PMID: 18083145
During the early phase of learning metallothionein-2 null mice were more susceptible to mercury-induced impairment compared to wildtype mice PMID: 18226494

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Protein Families

Metallothionein superfamily, Type 1 family

Database Links

KEGG: mmu:17750

STRING: 10090.ENSMUSP00000034214

UniGene: Mm.147226

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Code	CSB-E13693m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.