Mouse Mucin-2,MUC2 ELISA Kit

Code CSB-E15065m
Size 96T,5×96T,10×96T
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Product Details

Target Name
mucin 2, oligomeric mucus/gel-forming
Alternative Names
Muc2 ELISA Kit; Mucin-2 ELISA Kit; MUC-2 ELISA Kit; Colonic mucin ELISA Kit; MCM ELISA Kit; Secreted gel-forming mucin ELISA Kit; Fragments ELISA Kit
Abbreviation
MUC2
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:20Average %90
Range %83-97
1:40Average %95
Range %86-107
1:80Average %95
Range %86-102
1:160Average %95
Range %88-103
Recovery
The recovery of mouse MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9789-103
EDTA plasma (n=4)9389-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.113 2.024 2.069 1.916
10001.626 1.685 1.656 1.503
5001.105 1.166 1.136 0.983
2500.643 0.673 0.658 0.505
1250.458 0.481 0.470 0.317
62.50.319 0.337 0.328 0.175
31.250.252 0.262 0.257 0.104
00.152 0.154 0.153
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse MUC2 ELISA Kit was designed for the quantitative measurement of Mouse MUC2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

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Target Background

Function
(From Uniprot)
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
Subcellular Location
Secreted. Note=In the intestine, secreted into the inner and outer mucus layers.
Tissue Specificity
Highly expressed in goblet cells of the colon with lower levels in the small intestine and no expression in the stomach (at protein level).
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