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Mouse Pancreatic Lipase(PL)ELISA Kit

Citations (4) Protocol MSDS
Code CSB-E16930m
Size 96T,5×96T,10×96T
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Product Details

Target Name
Pancreatic Lipase(PL)
Alternative Names
PnlipPancreatic triacylglycerol lipase ELISA Kit; PL ELISA Kit; PTL ELISA Kit; Pancreatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit
Abbreviation
PL
Uniprot No.
Q6P8U6
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 mU/mL-4000 mU/mL
Sensitivity
15.6 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

CUSABIO's mouse pancreatic lipase (PL) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse PL in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of PL in samples. The color intensity is measured at 450 nm via a microplate reader.

PL is usually secreted by the pancreatic acinar cells and transferred to the duodenum to participate in the hydrolysis and digestion of fat, cholesterol esters, and fat-soluble vitamins. It is critical for the digestion and absorption of dietary fats and plays an important role in the regulation of gastric emptying and pancreatic and biliary secretion after ingestion of fat in humans. Plasma levels of pancreatic lipase are routinely measured in the diagnosis of pancreatic dysfunction. Congenital pancreatic lipase deficiency is a rare autosomal recessive exocrine pancreatic failure featured with reduced absorption of dietary fat and greasy voluminous stools, but apparent normal development and an overall good state of health.

Citations

Related Products

Pnlip Antibodies

Pnlip Proteins

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Target Background

Function
(From Uniprot)
Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
Gene References into Functions
  1. the ability to degrade unfolded protein decreases in the aging pancreas, and this leads to reduction of pancreatic lipase activity and a decrease in lipid absorption PMID: 25033985
  2. pancreatic triglyceride lipase has a critical role in dietary cholesterol absorption and a minimal role in dietary fat absorption in mice PMID: 12915407
  3. PTL and CEL serve complementary functions, working together to mediate the absorption of a major portion of dietary fat and fat-soluble vitamin esters PMID: 17604277
Subcellular Location
Secreted.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Pancreas.
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