Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cardiovascular
Alternative Names
PnlipPancreatic triacylglycerol lipase; PL; PTL; Pancreatic lipase; EC 3.1.1.3
Species
Mus musculus (Mouse)
Expression Region
17-465aa
Target Protein Sequence
REVCFDKLGCFSDDAPWSGTLDRPLKALPWSPAQINTRFLLYTNENPDNYQLITSDASNIRNSNFRTNRKTRIIIHGFIDKGEENWLSDMCKNMFRVESVNCICVDWKGGSRTTYTQATQNVRVVGAEVALLVNVLQSDLGYSLNNVHLIGHSLGSHIAGEAGKRTFGAIGRITGLDPAEPYFQGTPEEVRLDPTDAQFVDAIHTDAGPIIPNLGFGMSQTVGHLDFFPNGGIEMPGCQKNILSQIVDIDGIWEGTRNFAACNHLRSYKFYTDSIVNPTGFAGFSCSSYSLFTANKCFPCGSGGCPQMGHYADRYPGKTSRLYQTFYLNTGDKSNFARWRYQVTVTLSGQKVTGHILVSLFGNGGNSKQYEVFKGSLQPGTSHVNEFDSDVDVGDLQKVKFIWYNNVINPTLPKVGASRITVERNDGRVFNFCSQETVREDVLLTLSPC
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Mouse Pnlip contains amino acids 17-465. The expected molecular weight for the Pnlip protein is calculated to be 65.8 kDa. This protein is generated in a e.coli-based system. Fusion of the N-terminal 6xHis-SUMO tag into the Pnlip encoding gene fragment was conducted, allowing for easier detection and purification of the Pnlip protein in subsequent stages.
Mouse pancreatic triacylglycerol lipase (Pnlip) is a key enzyme involved in lipid digestion. Its main function includes hydrolyzing dietary triacylglycerols into fatty acids and glycerol, facilitating their absorption in the small intestine. In nutritional research, Pnlip contributes to understanding lipid metabolism and digestion. In gastrointestinal physiology, Pnlip plays a crucial role in maintaining lipid homeostasis. Investigating Pnlip provides insights into pancreatic function and its impact on overall metabolic health. Additionally, Pnlip's involvement in lipid metabolism makes it relevant in obesity and metabolic disorders research. Understanding the functions of Pnlip offers potential applications in designing therapeutic strategies targeting lipid-related diseases and metabolic disorders.
