Recombinant Mouse Pancreatic triacylglycerol lipase (Pnlip)

The region for expressing recombinant Mouse Pnlip contains amino acids 17-465. The expected molecular weight for the Pnlip protein is calculated to be 65.8 kDa. This protein is generated in a e.coli-based system. Fusion of the N-terminal 6xHis-SUMO tag into the Pnlip encoding gene fragment was conducted, allowing for easier detection and purification of the Pnlip protein in subsequent stages.

Mouse pancreatic triacylglycerol lipase (Pnlip) is a key enzyme involved in lipid digestion. Its main function includes hydrolyzing dietary triacylglycerols into fatty acids and glycerol, facilitating their absorption in the small intestine. In nutritional research, Pnlip contributes to understanding lipid metabolism and digestion. In gastrointestinal physiology, Pnlip plays a crucial role in maintaining lipid homeostasis. Investigating Pnlip provides insights into pancreatic function and its impact on overall metabolic health. Additionally, Pnlip's involvement in lipid metabolism makes it relevant in obesity and metabolic disorders research. Understanding the functions of Pnlip offers potential applications in designing therapeutic strategies targeting lipid-related diseases and metabolic disorders.