Name: Mouse Phenylalanine-4-hydroxylase(PAH) ELISA kit
Brand: CUSABIO
SKU: CSB-EL017396MO

Product Details

Target Name

phenylalanine hydroxylase

Alternative Names

Pah ELISA Kit; Phenylalanine-4-hydroxylase ELISA Kit; PAH ELISA Kit; EC 1.14.16.1 ELISA Kit; Phe-4-monooxygenase ELISA Kit

Abbreviation

PAH

Uniprot No.

P16331

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

23.5 pg/mL-1500 pg/mL

Sensitivity

5.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	101-109
1:2	Average %	95
	Range %	91-99
1:4	Average %	86
	Range %	83-89
1:8	Average %	92
	Range %	87-96

Recovery

The recovery of mouse PAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	89	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.646	2.701	2.674	2.481
750	2.063	2.000	2.032	1.839
375	1.324	1.405	1.365	1.172
187.5	0.826	0.834	0.830	0.637
94	0.593	0.587	0.590	0.397
47	0.362	0.371	0.367	0.174
23.5	0.278	0.284	0.281	0.088
0	0.192	0.194	0.193

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse PAH ELISA Kit was designed for the quantitative measurement of Mouse PAH protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Our customer would like to know if you have cross-reactivity data for the CSB-EL017396MO PAH ELISA kit (Mouse).
They are curious to know whether you have tested to see if this mouse kit cross-reacts with human.
Thanks for your assistance.

Thanks for your inquiry!
For different species, the primary structure and the advanced structure of proteins are different. The antibody's characteristics are also different. So we didn't validate human samples with mouse kit before. We don't suggest you use this kit to test human samples. Sorry we don't have human pah ELisa kit.

What is the contents of the antibody in this kit?

Thanks for your inquiry!
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody.

Target Background

Function
(From Uniprot)

Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.

Gene References into Functions

We propose that CNS monoamine deficiency may be the cause of the partially reversible adverse behavioral effects associated with chronic HPA in Pah(enu2) mice, but that phenylalanine-reducing treatments initiated during adulthood are unable to correct the neuropathology and attendant cognitive deficits that develop during juvenile life in late-treated Pah(enu2/enu2) mice PMID: 29331172
we assessed DNA methylation patterns in brain tissues using methylated DNA immunoprecipitation and paired end sequencing in adult PAH(enu2) animals maintained under either continuous dietary Phe restriction or chronic hyperphenylalaninemia. Heterozygous PAH(enu2/WT) litter mates served as controls for normal Phe exposure PMID: 26822703
These observations support the proposition that differences in phenylalanine hydroxylase activity underlie the variation in S-carboxymethyl-L-cysteine sulfoxidation and that no other enzyme is able to undertake this reaction. PMID: 26338263
A single injection of PAH-RBCs was able to decrease Phe levels by nearly 80% in normal mice PMID: 23867524
high levels of Phe, such as in phenylketonuria, are unlikely to directly induce brain hypomyelination, suggesting involvement of alternative mechanisms in this myelination defect PMID: 20151197
phenylalanine concentration regulates the activity of PAH in the hph-1 mouse and that this acts via a mechanism that includes phosphorylation of the PAH molecule PMID: 19560382
Tetrahydrobiopterin protects PAH activity in vivo. PMID: 15556637
low level of phenylalanine clearance was observed in mice expressing PAH and GTP-CH from the keratin 14 promoter PMID: 15946242

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Involvement in disease

Mouse strains deficient in phenylalanine hydroxylase (Pah) were created as models of phenylketonuria (PKU).

Protein Families

Biopterin-dependent aromatic amino acid hydroxylase family

Database Links

KEGG: mmu:18478

STRING: 10090.ENSMUSP00000020241

UniGene: Mm.263539

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Code	CSB-EL017396MO
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Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Mouse Phenylalanine-4-hydroxylase(PAH) ELISA kit

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Target Background