Mouse Platelet-derived growth factor DD(PDGF-DD) ELISA kit

Code CSB-EL017711MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
platelet derived growth factor DD
Abbreviation
PDGF-DD
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-96
1:2 Average % 102
Range % 98-106
1:4 Average % 88
Range % 84-93
1:8 Average % 100
Range % 95-104
Recovery
The recovery of mouse PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 86 83-89
EDTA plasma (n=4) 97 93-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.548 2.601 2.575 2.448
5 2.115 2.012 2.064 1.937
2.5 1.357 1.303 1.330 1.203
1.25 0.786 0.732 0.759 0.632
0.625 0.459 0.462 0.461 0.334
0.312 0.292 0.289 0.291 0.164
0.156 0.211 0.204 0.208 0.081
0 0.126 0.128 0.127  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse PDGF-DD ELISA Kit was designed for the quantitative measurement of Mouse PDGF-DD protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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