Product Details

Target Name

polymeric immunoglobulin receptor

Alternative Names

Pigr; Polymeric immunoglobulin receptor; PIgR; Poly-Ig receptor

Abbreviation

PIGR

Uniprot No.

O70570

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PIGR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	85-94
1:200	Average %	89
	Range %	85-93
1:400	Average %	84
	Range %	80-88
1:800	Average %	102
	Range %	98-109

Recovery

The recovery of mouse PIGR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	91	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.925	1.867	1.896	1.757
1000	1.366	1.282	1.324	1.185
500	0.872	0.849	0.861	0.722
250	0.551	0.546	0.549	0.410
125	0.415	0.428	0.422	0.283
62.5	0.284	0.291	0.288	0.149
31.25	0.231	0.224	0.228	0.089
0	0.142	0.135	0.139

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse PIGR ELISA Kit was designed for the quantitative measurement of Mouse PIGR protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Background

Function
(From Uniprot)

Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.; Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens. On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells.

Gene References into Functions

pIgR/SIgA deficiency in the airways leads to persistent activation of innate immune responses to resident lung microbiota, driving progressive small airway remodelling and emphysema. PMID: 27046438
Increased autoimmune diabetes in Pigr-deficient NOD mice is due to a "Hitchhiking" interval that refines the genetic effect of Idd5.4. PMID: 25835383
the polymeric immunoglobulin receptor (pIgR) is highly expressed by renal cyst-lining cells. pIgR expression is, in part, driven by aberrant STAT6 pathway activation. PMID: 25922073
pIgR on the blood-brain barrier endothelium may represent a novel pneumococcal adhesion receptor. PMID: 24841255
Results reveal a novel Yes-EGFR-ERK-FIP5 signalling network for regulation of pIgA-pIgR transcytosis. PMID: 21037565
Blockade of transepithelial transport of dimeric IgA in pIgR knockout mice reduces the degree of protection or cross-protection against viral infection, in parallel with a marked increase in serum IgA concentration and decrease in nasal IgA concentration. PMID: 11884464
Mice deficient in SIgA have been generated by knocking out the pIgR and assessing their ability to maintain normal mucosal homeostasis and to induce mucosal and systemic immune responses against pathogenic and nonpathogenic stimuli. PMID: 12594279
Mice that lack expression of the polymeric immunoglobulin receptor have approximately 5-fold more systemic IgA and 6-fold less nasal IgA antibodies than wild-type mice due to defective IgA transport into mucosal tissues. PMID: 15383591
Data suggest that the pIgR and/or the secretory component are important for the maintenance of epithelial integrity and mucosal homeostasis in the colonic epithelium. PMID: 16911679
pIgR is essential in intestinal defense against pathogenic microbes with high-level and persistent luminal presence. PMID: 17056558
Novel localization of pIgR to the regulated secretory pathway of lacrimal gland acinar cells and its secretion therefrom may be affected by its novel interaction with Rab3D. PMID: 18171724
The poly immunoglobulin receptor is the main pathway for IgA transport into the male reproductive tract. PMID: 18803626

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Subcellular Location

[Polymeric immunoglobulin receptor]: Cell membrane; Single-pass type I membrane protein.; [Secretory component]: Secreted.

Database Links

KEGG: mmu:18703

STRING: 10090.ENSMUSP00000027675

UniGene: Mm.276414

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Code	CSB-EL017981MO
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Mouse Polymeric immunoglobulin receptor(PIGR) ELISA kit

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