Mouse Protein Wnt-16(WNT16) ELISA kit

Instructions
Code CSB-EL026132MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name wingless-type MMTV integration site family, member 16
Alternative Names Wnt16 ELISA Kit; Protein Wnt-16 ELISA Kit
Abbreviation WNT16
Uniprot No. Q9QYS1
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %103
Range %101-105
1:2Average %96
Range %93-99
1:4Average %89
Range %86-92
1:8Average %98
Range %94-102
Recovery
The recovery of mouse WNT16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 106101-111
EDTA plasma (n=4)9289-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.379 2.465 2.422 2.268
20002.022 1.970 1.996 1.842
10001.450 1.560 1.505 1.351
5000.865 0.899 0.882 0.728
2500.531 0.527 0.529 0.375
1250.375 0.383 0.379 0.225
62.50.288 0.278 0.283 0.129
00.158 0.150 0.154
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Citations

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Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Gene References into Functions
  1. A large number of Mef2c targets overlapped with genes down-regulated by Wnt16 and Mef2c itself was transcriptionally repressed by Wnt16 suggesting that Mef2c plays a role in Wnt16-mediated transcriptional regulation. PMID: 29981832
  2. that WNT16 plays a critical role for acquisition of both cortical and trabecular bone mass and strength PMID: 28013361
  3. WNT16 antagonises excessive canonical WNT activation and protects cartilage in osteoarthritis. PMID: 27147711
  4. Wnt16 Is Associated with Age-Related Bone Loss and Estrogen Withdrawal in Murine Bone PMID: 26451596
  5. Describe TGFbeta-Wnt16-Notch signaling conduit in the chondrocyte-like transformation of VSMCs and identify endogenous TGFbeta activity in MGP-null VSMCs as a critical mediator of chondrogenesis. PMID: 25614285
  6. Wnt5a abrogated the inhibitory effects of Wnt16 on Rankl-induced osteoclastogenesis PMID: 26093292
  7. These findings suggest that WNT16 acting via canonical WNT signaling regulates mechanical strain-induced periosteal bone formation and bone size. PMID: 25521583
  8. Osteoblast-derived WNT16 is a previously unreported key regulator of osteoclastogenesis and fracture susceptibility. PMID: 25306233
  9. Wnt16 is involved in intramembranous ossification and suppresses osteoblast differentiation through the Wnt/beta-catenin pathway. PMID: 24037946
  10. Study revealed new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. PMID: 19185060

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Subcellular Location Secreted, extracellular space, extracellular matrix.
Protein Families Wnt family
Database Links

KEGG: mmu:93735

STRING: 10090.ENSMUSP00000031681

UniGene: Mm.137403

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