Mouse Protein notum homolog(NOTUM) ELISA kit

Code CSB-EL015955MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
notum pectinacetylesterase homolog (Drosophila)
Alternative Names
NotumPalmitoleoyl-protein carboxylesterase NOTUM ELISA Kit; EC ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates
Detection Range
0.156 ng/mL-10 ng/mL
0.039 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 109  
Range % 104-113  
1:2 Average % 93  
Range % 89-99  
1:4 Average % 100  
Range % 96-104  
1:8 Average % 88  
Range % 84-92  
The recovery of mouse NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 81-89  
EDTA plasma (n=4) 98 94-102  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.779 2.798 2.789 2.587  
5 2.201 2.156 2.179 1.977  
2.5 1.484 1.451 1.468 1.266  
1.25 0.845 0.899 0.872 0.670  
0.625 0.555 0.561 0.558 0.356  
0.312 0.432 0.445 0.439 0.237  
0.156 0.312 0.326 0.319 0.117  
0 0.201 0.203 0.202    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse NOTUM ELISA Kit was designed for the quantitative measurement of Mouse NOTUM protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

(From Uniprot)
Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
Gene References into Functions
  1. Notum influences mesenchymal cell differentiation by generating a barrier for Wnt ligands produced and secreted by airway epithelial cells to attenuate Wnt signaling. PMID: 29428562
  2. The findings support a significant role for NOTUM in modulating WNT signaling pathways that have pleiotropic effects on tooth and kidney development. PMID: 26926082
  3. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
  4. Male mice deficient in Notum specifically in the liver develop metabolic dysfunctions implicating Notum in the development of Type 2 diabetes. PMID: 26974334
  5. Describes the human ortholog, and analyzes both the human and mouse promoters. PMID: 18429952

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Subcellular Location
Protein Families
Pectinacetylesterase family, Notum subfamily
Tissue Specificity
Widely expressed. Expressed in lung, ovary, kidney, liver and brain. Not detected in thymus, heart, spleen, stomach, skeletal muscle and bone marrow.
Database Links
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