Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

Code CSB-E12639m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
surfactant protein C
Alternative Names
Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SP5 ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
2.3 ng/mL-150 ng/mL
0.58 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %86
Range %80-92
1:2Average %91
Range %85-96
1:4Average %95
Range %90-100
1:8Average %98
Range %92-102
The recovery of mouse SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-98
EDTA plasma (n=4)9690-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
1502.147 2.052 2.100 2.009
751.693 1.576 1.635 1.544
37.51.121 1.044 1.083 0.992
18.80.648 0.639 0.644 0.553
9.40.429 0.424 0.427 0.336
4.70.295 0.279 0.287 0.196
2.30.194 0.179 0.187 0.096
00.092 0.090 0.091  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

CUSABIO's mouse pulmonary surfactant-associated protein C (SP-C) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SP-C in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of SP-C in samples. The color intensity is measured at 450 nm via a microplate reader.

SP-C (SFTPC) is expressed only in the lung and is a highly specific marker for identifying TII cells. SP-C has several functions in pulmonary surfactant, including increasing the adsorption and spreading of phospholipids at the air-liquid interface thereby promoting the surface tension-lowering properties of surfactant, a role in surfactant recycling and homeostasis, and involvement in the modulation of the innate defense system. Mutations in SP-C protein and its total absence can lead to interstitial lung disease.

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Target Background

(From Uniprot)
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Subcellular Location
Secreted, extracellular space, surface film.
Database Links
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