Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

Code CSB-E12639m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name surfactant protein C
Alternative Names Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SP5 ELISA Kit
Abbreviation SFTPC
Uniprot No. P21841
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 2.3 ng/mL-150 ng/mL
Sensitivity 0.58 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %86
Range %80-92
1:2Average %91
Range %85-96
1:4Average %95
Range %90-100
1:8Average %98
Range %92-102
The recovery of mouse SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-98
EDTA plasma (n=4)9690-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
1502.147 2.052 2.100 2.009
751.693 1.576 1.635 1.544
37.51.121 1.044 1.083 0.992
18.80.648 0.639 0.644 0.553
9.40.429 0.424 0.427 0.336
4.70.295 0.279 0.287 0.196
2.30.194 0.179 0.187 0.096
00.092 0.090 0.091  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Subcellular Location Secreted, extracellular space, surface film.
Database Links

STRING: 10090.ENSMUSP00000022692

UniGene: Mm.24040


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