24T ELISA Kit Trial Size (Only USD$150/ kit)
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|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of mouse SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||96||90-100|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
CUSABIO's mouse pulmonary surfactant-associated protein C (SP-C) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SP-C in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of SP-C in samples. The color intensity is measured at 450 nm via a microplate reader.
SP-C (SFTPC) is expressed only in the lung and is a highly specific marker for identifying TII cells. SP-C has several functions in pulmonary surfactant, including increasing the adsorption and spreading of phospholipids at the air-liquid interface thereby promoting the surface tension-lowering properties of surfactant, a role in surfactant recycling and homeostasis, and involvement in the modulation of the innate defense system. Mutations in SP-C protein and its total absence can lead to interstitial lung disease.
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