Mouse R-spondin-1(RSPO1) ELISA kit

Product Details

Customer Reviews and Q&A

Target Background

Product Details

Target Name

R-spondin homolog (Xenopus laevis)

Alternative Names

Rspo1R-spondin-1 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 3 ELISA kit; Cristin-3 ELISA kit; mCristin-3 ELISA kit; Roof plate-specific spondin-1 ELISA kit

Abbreviation

RSPO1

Uniprot No.

Q9Z132

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Developmental Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	101
	Range %	96-105
1:4	Average %	93
	Range %	85-97
1:8	Average %	92
	Range %	84-96

Recovery

The recovery of mouse RSPO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	81-94
EDTA plasma (n=4)	107	101-111

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.198	2.113	2.156	1.986
200	1.312	1.276	1.294	1.124
100	0.886	0.844	0.865	0.695
50	0.504	0.531	0.518	0.348
25	0.402	0.424	0.413	0.243
12.5	0.319	0.333	0.326	0.156
6.25	0.237	0.231	0.234	0.064
0	0.171	0.169	0.170

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse RSPO1 ELISA Kit was designed for the quantitative measurement of Mouse RSPO1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Background

Function
(From Uniprot)

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1.

Gene References into Functions

reciprocal control of antagonistic Wnt signaling pathways by Rspo1 in muscle stem cell progeny is a key step ensuring normal tissue architecture restoration following acute damage. PMID: 28273449
In Rspo1 knock-out mice during pregnancy, a stronger expression of mesenchymal markers was observed, without modifications to the structure of mammary epithelial tissue. Mammary epithelial cell immunohistochemical analysis revealed a persistence of virgin markers, which signify a delay in cell differentiation. PMID: 27611670
We also show that R-spondin1 is depleted in RANK-null progenitors, and that its exogenous administration rescues key aspects of RANK deficiency by reinstating a WNT response and mammary cell expansion PMID: 26095608
R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis PMID: 25910078
The ascl2 is regulated in a direct autoactivatory loop, leading to a distinct on/off expression pattern, and Wnt/R-spondin can activate this regulatory loop. PMID: 25620640
RSPO1, WNT4, and beta-catenin have roles in the signaling pathway during ovarian differentiation in mice. PMID: 25187620
Rspo1 is required for ovarian differentiation by activation of the WNT/CTNNB1 signalling pathway PMID: 18250098
Rspo1 is involved in maintenance of the immature state of the progenitors of the granulosa cells of the ovary PMID: 24036309
Rspo1 is involved in the progenitor cells proliferation of the coelomic epithelium of the undifferentiated gonad of the mouse embryo PMID: 23095882
ZNRF3 binds RSPO1 and LGR5-RSPO1 with micromolar affinity via RSPO1 furin-like 1 (Fu1) domain. PMID: 24349440
overexpressed in culture-activated hepatic stellate cells PMID: 25218283
R-spondin1 (Rspo1) as a novel hormonal mediator in the mammary gland PMID: 25260709
Findings indicate that R-spondin1 (Rspo1) is a negative regulator of beta-cell neogenesis and suggest that Rspo1 may serve as a therapeutic target for the preservation or restoration of functional [beta]-cells in diabetes. PMID: 24263108
R-Spondin 1 is upregulated by mechanical signals and secreted. R-Spondin 1 promotes bone formation in three mouse models of osteoporosis. PMID: 23974989
Wnt/Rspondin/beta-catenin signals control axonal sorting and lineage progression in Schwann cell development. PMID: 24151333
In conclusion, present study highlights the role of Rspo 1 in bone remodeling where it activates Wnt signaling to induce differentiation, as shown in human as well murine in vitro osteoblast cell models. PMID: 23617070
Rspo1 is an essential activator of ovarian development not only in normal situations, but also in sex reversal situations PMID: 23300469
this study identifies Rspo1 and Wnt4 as two new regulators of cell proliferation in the early gonad regardless of its sex, in addition to the specific role of these genes in ovarian differentiation. PMID: 23095882
Foxl2 expression partly depends of Rspo1 signalisation. PMID: 22116255
RSPO1/beta-catenin signaling is involved in meiosis in fetal germ cells and contributes to the cellular decision of germ cells to differentiate into oocyte or sperm. PMID: 21991325
The study reveals a role for RSPO1 as a regulator of both beta cell proliferation and neogenesis in vivo, and reinforces the importance of cWNT signalling for the maintenance of normal pancreatic beta cell behaviour. PMID: 21484214
The Wnt agonist R-spondin1 regulates systemic graft-versus-host disease by protecting intestinal stem cells. PMID: 21282378
R-spondin-1 is a novel beta-cell growth factor and insulin secretagogue PMID: 20442404
Intestinal ischemia-reperfusion injury may inhibit expression of RSpo1 in the early stage, and enhance its expression in the middle stage. PMID: 19726353
R-spondin1, an intestinal stem cell growth factor, protects against radiation-induced gastrointestinal syndrome in mice PMID: 19956666
R-spondin might be a novel marker of the boundary between the roof plate and neuroepithelium and may contribute to the development of dorsal neural tube under the regulation of Wnts. PMID: 14732490
R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/beta-catenin signaling in the uncommitted mesenchymal C2C12 cells. PMID: 18242177
Rspo1 is required to fully suppress the male differentiation program and to maintain germ cell survival during the development of XX gonads. PMID: 18250097
Balance between Sox9 and beta-catenin activation determines the fate of the gonad, with Rspo1 acting as a crucial regulator of canonical beta-catenin signaling required for female development. PMID: 18250098
RSPO1 is up-regulated in the embryonic gonads of female vertebrates with different sex-determining mechanisms. PMID: 18651984
R-Spondin1 protects mice from chemotherapy or radiation-induced oral mucositis through the canonical Wnt/beta-catenin pathway PMID: 19179402
SRY represses the transcriptional of the Rspo1/Wnt target genes involved in ovarian determination. PMID: 19376480
these data demonstrate that local epithelial Rspo1 signalling is required for normal development of the mammary gland. PMID: 19857464

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Subcellular Location

Secreted. Nucleus.

Protein Families

R-spondin family

Tissue Specificity

Expressed in the dorsal part of the neural tube on 10 and 12 dpc, especially in the boundary region between roof plate and neuroepithelium. This expression is enhanced in the rostral part. Also expressed in other tissues such as truncal region neighboring

Database Links

KEGG: mmu:192199

STRING: 10090.ENSMUSP00000030687

UniGene: Mm.42202

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Code	CSB-EL020550MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.