Mouse R-spondin-3(RSPO3) ELISA kit

Product Details

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Target Background

Product Details

Target Name

R-spondin 3 homolog (Xenopus laevis)

Alternative Names

Rspo3 ELISA Kit; R-spondin-3 ELISA Kit; Cabriolet ELISA Kit; Cysteine-rich and single thrombospondin domain-containing protein 1 ELISA Kit; Cristin-1 ELISA Kit; Nucleopondin ELISA Kit; Roof plate-specific spondin-3 ELISA Kit

Abbreviation

RSPO3

Uniprot No.

Q2TJ95

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.7 pg/mL-300 pg/mL

Sensitivity

1.17 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Developmental Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	98
	Range %	93-103
1:4	Average %	102
	Range %	98-105
1:8	Average %	94
	Range %	92-96

Recovery

The recovery of mouse RSPO3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-100
EDTA plasma (n=4)	93	90-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.693	2.800	2.747	2.597
150	1.811	1.970	1.891	1.741
75	1.073	1.135	1.104	0.954
37.5	0.652	0.701	0.677	0.527
18.75	0.369	0.375	0.372	0.222
9.4	0.247	0.266	0.257	0.107
4.7	0.202	0.214	0.208	0.058
0	0.158	0.142	0.150

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse RSPO3 ELISA Kit was designed for the quantitative measurement of Mouse RSPO3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

Target Background

Function
(From Uniprot)

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.

Gene References into Functions

These results shed new light on the role of Rspo3 in heart development and demonstrate that LGR4 is the principal R-spondin 3 receptor in the heart. PMID: 29859889
Results identify a mechanism through which localized expression of RSPO3 induces proliferation of the coronary arteries at their stems and permits their formation. PMID: 28834739
Rspo3-LGR4 axis protects hepatocytes from hypoxia/reoxygenation injury via activating beta-catenin. PMID: 29555474
H. pylori infection increases stromal R-spondin 3 expression and expands the Axin2(+) cell pool to cause hyperproliferation and gland hyperplasia; the ability of stromal niche cells to control and adapt epithelial stem cell dynamics constitutes a sophisticated mechanism that orchestrates epithelial regeneration and maintenance of tissue integrity PMID: 28813421
We provide in vivo evidence that RSPO3 stimulates the crypt stem cell and niche compartments and drives rapid intestinal tumorigenesis. PMID: 27511199
Rspo3 function remains essential in adult life to ensure replenishment of lost cells and maintain the properties of the zona glomerulosa PMID: 27313319
Conditional deletion of Rspo3 in mice disrupts activation of central fate, demonstrating its crucial role in determining and maintaining beta-catenin-dependent zonation. PMID: 26655896
data identify endothelial RSPO3-driven non-canonical WNT/Ca(2+)/NFAT signaling as a critical maintenance pathway of the remodeling vasculature PMID: 26766444
Nkx2-5 has a role in regulating cardiac growth through modulation of Wnt signaling by R-spondin3 PMID: 25053429
Rspo3 loss of function mutation in combination with Rspo2 mutation,but not on its own, causes severe limb truncation. PMID: 22610508
Sexually dimorphic expression patterns were observed for WNT/beta-catenin-responsive genes, and for Wnt2b, Wnt4, Wnt7a, Wnt9b, Wnt10b, Wnt11, Wnt16, and Rspo3 mRNAs. PMID: 21936019
Data show that Rspo3 binds syndecan 4 and that together they activate Wnt5a/PCP signaling. PMID: 21397842
suggest a critical role for Rspo3 in the interaction between chorion and allantois in labyrinthine development PMID: 16963017
identify Rspo3 as a novel, evolutionarily conserved angiogenic factor in embryogenesis PMID: 18842812

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Subcellular Location

Secreted.

Protein Families

R-spondin family

Tissue Specificity

Highly expressed in endothelial cells.

Database Links

KEGG: mmu:72780

STRING: 10090.ENSMUSP00000090287

UniGene: Mm.45191

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Code	CSB-EL020552MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.