Mouse R-spondin-4(RSPO4) ELISA kit

Code CSB-EL020553MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
R-spondin family, member 4
Alternative Names
Rspo4R-spondin-4 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 4 ELISA kit; Cristin-4 ELISA kit; mCristin-4 ELISA kit; Roof plate-specific spondin-4 ELISA kit
Abbreviation
RSPO4
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
34.4 pg/mL-2200 pg/mL
Sensitivity
8.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Developmental Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 87-95
1:2 Average % 97
Range % 93-102
1:4 Average % 92
Range % 85-96
1:8 Average % 97
Range % 95-101
Recovery
The recovery of mouse RSPO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 92-101
EDTA plasma (n=4) 87 83-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2200 2.452 2.371 2.412 2.280
1100 1.956 1.815 1.886 1.754
550 1.208 1.182 1.195 1.063
275 0.717 0.701 0.709 0.577
137.5 0.425 0.413 0.419 0.287
68.75 0.292 0.288 0.290 0.158
34.4 0.196 0.192 0.194 0.062
0 0.132 0.131 0.132  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse RSPO4 ELISA Kit was designed for the quantitative measurement of Mouse RSPO4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 34.4 pg/mL-2200 pg/mL and the sensitivity is 8.6 pg/mL.

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Target Background

Function
(From Uniprot)
Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.
Subcellular Location
Secreted.
Protein Families
R-spondin family
Database Links
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