Mouse Regenerating islet-derived protein 3-alpha(REG3A) ELISA kit

Code CSB-EL019548MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
regenerating islet-derived 3 alpha
Alternative Names
Reg3a ELISA Kit; Pap2 ELISA Kit; Regenerating islet-derived protein 3-alpha ELISA Kit; REG-3-alpha ELISA Kit; Islet of Langerhans regenerating protein 3 ELISA Kit; Lithostathine 3 ELISA Kit; Pancreatitis-associated protein 2 ELISA Kit; Regenerating islet-derived protein III-alpha ELISA Kit; Reg III-alpha) [Cleaved into: Regenerating islet-derived protein 3-alpha 16.5 kDa form; Regenerating islet-derived protein 3-alpha 15 kDa form] ELISA Kit
Abbreviation
REG3A
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 102
Range % 98-106
1:2 Average % 98
Range % 96-104
1:4 Average % 88
Range % 86-95
1:8 Average % 96
Range % 91-102
Recovery
The recovery of mouse REG3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-97
EDTA plasma (n=4) 88 84-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.360 2.512 2.436 2.311
100 1.831 1.987 1.909 1.784
50 1.252 1.254 1.253 1.128
25 0.747 0.766 0.757 0.632
12.5 0.473 0.512 0.493 0.368
6.25 0.315 0.321 0.318 0.193
3.12 0.219 0.217 0.218 0.093
0 0.124 0.126 0.125  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse REG3A ELISA Kit was designed for the quantitative measurement of Mouse REG3A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

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Target Background

Function
(From Uniprot)
Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
Gene References into Functions
  1. skin injury increases IL-36gamma via the activation of TLR3-SLUG-VDR axis and IL-36gamma induces REG3A to promote wound healing PMID: 28774595
  2. Reg3alpha is an extracellular matrix-targeted reactive oxygen species scavenger that binds the fibrin scaffold resulting from hepatocyte death during acute liver failure. PMID: 25938566
  3. Data show that regenerating islet-derived 3-alpha (REG3alpha) is a plasma biomarker of gastrointestinal tract graft-versus-host disease that can be combined with clinical stage and histologic grade to improve risk stratification of patients. PMID: 21979939
  4. Reg3alpha stimulates beta-cell replication, by activating Akt kinase and increasing the levels of cyclin D1/CDK4. PMID: 19343564
Involvement in disease
Overexpressed during the acute phase of pancreatitis.
Subcellular Location
Secreted.
Tissue Specificity
Small intestine and pancreas.
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