Mouse Resistin ELISA Kit

Code CSB-E06886m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
resistin
Alternative Names
Retn ELISA Kit; Fizz3 ELISA Kit; Resistin ELISA Kit; Adipose tissue-specific secretory factor ELISA Kit; ADSF ELISA Kit; Adipose-specific cysteine-rich secreted protein A12-alpha ELISA Kit; Cysteine-rich secreted protein FIZZ3 ELISA Kit
Abbreviation
RETN
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
41.036 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %91-103
1:200Average %85
Range %80-90
1:400Average %93
Range %89-98
1:800Average %100
Range %94-105
Recovery
The recovery of mouse resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.524 2.485 2.505 2.318
20001.917 2.007 1.962 1.775
10001.294 1.352 1.323 1.136
5000.893 0.849 0.871 0.684
2500.627 0.601 0.614 0.427
1250.421 0.447 0.434 0.247
62.50.297 0.285 0.291 0.104
00.190 0.183 0.187
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse RETN ELISA Kit was designed for the quantitative measurement of Mouse RETN protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 41.036 pg/mL.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Mouse

Sample dilution: 1:100

Review: I used CSB-E06886m to detect the serum of C57 mice induced by high-fat diet, including normal diet, high-fat induced model group and high-fat induced administration group, and the dilution ratio was 1: 200. The experimental process is simple and easy to learn, easy to use, and the test results are accurate!

By Anonymous

Target Background

Function
(From Uniprot)
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Gene References into Functions
  1. Resistin inhibited the activation of CREB, resulting in suppression of PGC-1alpha. Importantly, overexpressing PGC-1alpha can rescue the inhibitory effects of resistin on fatty acid beta oxidation. PMID: 29730652
  2. Resistin-associated VSMC dysfunction and intimal hyperplasia are related to PKCepsilon-dependent Nox activation and ROS generation PMID: 27573736
  3. OLI is a physiological repressor of systemic resistin release whereas FFA upregulate resistin release in vitro from adipocytes. PMID: 26285069
  4. Resistin possibly acts via an intracrine pathway as an intracellular sensor, regulating the adipocyte insulin sensitivity. PMID: 26036220
  5. Mouse Resistin has regulative effects on murine bone marrow hematopoiesis. PMID: 26572487
  6. murine resistin is increased in the lungs of wild-type mice following acute ozone exposure but does not promote ozone-induced lung pathology. PMID: 26386120
  7. Inhibition of miR-696 restored the triglycerides content by up to 80%, which suggests that, in C2C12 cells, resistin at least partially increases the deposition of lipids through miR-696. PMID: 25962325
  8. Antiresistin RNA oligo ameliorated metabolic abnormalities and histopathology of nonalcoholic fatty liver disease through attenuating proinflammatory cytokines PMID: 25922835
  9. Resistin regulates PAI-1 expression in 3T3-L1 adipocytes via Akt phosphorylation. PMID: 24667608
  10. Oxidized-LDL promotes the expression and secretion of visfatin and resistin through its activation of endoplasmic reticulum stress. PMID: 24278099
  11. ET type-A receptor, ERK1/2, JNKs, AKT, and JAK2, but not ET type-B receptor or p38, are necessary for the ET-1 stimulation of resistin gene expression PMID: 24424064
  12. resistin is a negative regulator of SIRT1 in both human hepatoma cell line HepG2 and mouse hepatocytes. PMID: 23827175
  13. Both resistin gene expression and its secretion from the epididymal adipose tissue were decreased in db/db obese mice, while the insulin-sensitizing drug rosiglitazone increased resistin production. PMID: 23776497
  14. lipid content was significantly decreased in 3T3-L1 cells transfected with anti-resistin small interfering RNA (siRNA) after adipocyte differentiation PMID: 24034627
  15. The effect between resistin and adiponectin on the proliferation of vascular smooth muscle cells (VSMCs), was investigated. PMID: 23267839
  16. The current study was initiated to determine whether resistin has additional roles in glucose-dependent insulinotropic polypeptide -regulated adipocyte functions. PMID: 23002036
  17. significant changes in fasting-induced adipose factor (FIAF), resistin (Rstn), long-form leptin receptor (LepRb) and suppressor of cytokine-3 (SOCS3) gene expression following chronic SCI and with advanced age PMID: 22815920
  18. results identify resistin as a potential inhibitory ligand for the receptor ROR1 and demonstrate, for the first time, that ROR1 plays an important role in adipogenesis and glucose homeostasis in 3T3-L1 cellsand glucose homeostasis in 3T3-L1 cells. PMID: 22074948
  19. Cleavage product of decorin serves as a functional receptor of resistin in adipocyte progenitors and may regulate white adipose tissue expansion. PMID: 21683670
  20. review of evidence that resistin (secreted by adipocytes in the mouse) is an inflammatory biomarker and a potential mediator of obesity, diabetes and cardiovascular disease [REVIEW] PMID: 21497511
  21. Valsartan did not alter resistin expression in the ob/ob mouse. PMID: 20472602
  22. Data suggest that s-resistin isoform may play a role in inflammatory processes. PMID: 20627112
  23. data suggest that endogenous resistin might be capable of fine-tuning the expression and enzymatic activity of various hypothalamic targets previously implicated in the delicate homeostatic control of food intake PMID: 19646421
  24. Tumor necrosis factor alpha is a negative regulator of resistin gene expression and secretion in 3T3-L1 adipocytes PMID: 11689013
  25. Mechanisms regulating adipocyte expression of resistin PMID: 11901161
  26. examined the effect on resistin expression of various hormones and cytokines known to affect insulin sensitivity. PMID: 12031960
  27. Data demonstrate that basal and hormonal stimulation of resistin secretion by insulin is inhibited by ET-1. PMID: 12163029
  28. results indicate that the substantial reduction of adiposity in lactation does not lead to any changes in resistin gene expression in white adipose tissue PMID: 12163041
  29. expression and regulation in mouse pituitary; data suggest that local resistin expression could have functional implications during prepubertal maturation of the hypothalamic-pituitary system PMID: 12208498
  30. during in vivo bone formation and remodeling, probably acting as a co-factor or accessory protein that modulates the effects of primary signaling molecules PMID: 12413946
  31. Resistin expression is regulated by C-EBP Proteins, peroxisome proliferator-activated receptor, and signal-transducing molecules. PMID: 12437985
  32. Resistin is regulated and expressed in mouse brown adipocytes PMID: 12482590
  33. resistin does not alter insulin receptor signaling but does affect insulin-stimulated glucose uptake, presumably by decreasing the intrinsic activity of cell surface glucose transporters PMID: 12618360
  34. ADSF/resistin secretion controls fat cell differentiation and adipose tissue development. PMID: 12660879
  35. Despite severe weight loss and significant falls in leptin expression and insulin concentration, resistin gene expression appears unchanged in white adipose tissue of mice with MAC16 tumour. PMID: 12660881
  36. Data show that neuropeptie Y significantly increased resistin mRNA expression in white adipose tissue compared with artificial cerebrospinal fluid treated controls. PMID: 12684710
  37. resistin is a novel secreted factor first identified as a TZD-suppressible gene PMID: 12700889
  38. results demonstrate that leptin decreases resistin expression and suggest that resistin may influence glucose homeostasis PMID: 14962997
  39. mice lacking resistin exhibit low blood glucose levels after fasting, due to reduced hepatic glucose production; data support a physiological function for resistin in the maintenance of blood glucose during fasting PMID: 14976316
  40. Inhibits insulin-stimulaed glucose uptake into skeletal muscle cells without affecting insulin signaling pathways. Review. PMID: 15055467
  41. demonstrates in vivo the inhibitory role of ADSF in adipogenesis PMID: 15090646
  42. Pituitary resistin expression is age- and gender-dependent. In ob/ob and fasted mice, resistin is regulated in a tissue-specific manner. Visceral fat obesity increases but starvation decreases resistin mRNA. PMID: 15103228
  43. Plasma resistin of leptin-treated mice was barely decreased. PMID: 15126241
  44. Chronic hyperresistinemia impairs normal glucose metabolism. PMID: 15189975
  45. Nutritional regulation of resistin and changes in resistin gene expression and circulating levels in obesity are mediated, at least in part, through insulin and glucose. PMID: 15220189
  46. results indicate that chronic "hyper-resistinemia" leads to whole-body insulin resistance involving impaired insulin signaling in skeletal muscle, liver, and adipose tissue, resulting in glucose intolerance, hyperinsulinemia, and hypertriglyceridemia PMID: 15254589
  47. These results provide strong support for a physiological role of resistin in the development of hepatic insulin resistance. PMID: 15254590
  48. Article reviews recent studies of Resistin/ADSF/FIZZ3 in transgenic and knockout mice. PMID: 15358276
  49. Resistin overexpression induces dyslipidemia in mice, which is commonly seen in the insulin-resistant state, partially through enhanced secretion of lipoproteins. PMID: 15471967
  50. gender differences in resistin, adiponectin and leptin mRNA expression and plasma levels were not ablated by castration and seem to be dependent on other factors in addition to gonadal steroids PMID: 15483213

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Resistin/FIZZ family
Tissue Specificity
Expressed in white but not brown adipose tissue in a variety of organs.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1