Product Details

Target Name

retinol binding protein 4, plasma

Alternative Names

Rbp4 ELISA Kit; Retinol-binding protein 4 ELISA Kit; Plasma retinol-binding protein ELISA Kit; PRBP ELISA Kit; RBP ELISA Kit

Abbreviation

RBP4

Uniprot No.

Q00724

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, urine, tissue homogenates

Detection Range

0.312 μg/mL-20 μg/mL

Sensitivity

0.078 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	95
	Range %	90-103
1:4	Average %	88
	Range %	85-93
1:8	Average %	87
	Range %	83-92

Recovery

The recovery of mouse RBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	93-104
EDTA plasma (n=4)	94	90-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
20	0.157	0.145	0.151
10	0.259	0.264	0.262
5	0.369	0.385	0.377
2.5	0.587	0.545	0.566
1.25	0.830	0.823	0.827
0.625	1.278	1.233	1.256
0.312	1.646	1.696	1.671
0	2.622	2.745	2.684

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This mouse RBP4 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse RBP4 in multiple samples, including serum, plasma, urine, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the RBP4 levels in the sample and is measured at 450 nm through a microplate reader.

RBP4 is the major transporter of the hydrophobic molecule retinol or vitamin A in circulation. It transports retinol from the liver to the peripheral tissues. Plasma RBP4 levels positively correlate with retinol levels. RBP4 also transports adipokine and fatty acid. Studies have found that RBP4 functions as a hepatokine and mediates the effects of the molecular clock on glucose metabolism in mice. RBP4 is implicated in a variety of human conditions that include impaired vision and ocular diseases, disorders of glucose and lipid homeostasis, and cardiovascular diseases.

Code	CSB-E09151m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Mouse Retinol-binding protein,RBP ELISA Kit

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