Mouse SPARC-like protein 1(SPARCL1) ELISA kit

Instructions
Code CSB-EL022487MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name SPARC-like 1 (hevin)
Alternative Names Sparcl1 ELISA Kit; Ecm2 ELISA Kit; Sc1 ELISA Kit; SPARC-like protein 1 ELISA Kit; Extracellular matrix protein 2 ELISA Kit; Matrix glycoprotein Sc1 ELISA Kit
Abbreviation SPARCL1
Uniprot No. P70663
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPARCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %86-93
1:2Average %95
Range %90-101
1:4Average %94
Range %90-97
1:8Average %97
Range %95-100
Recovery
The recovery of mouse SPARCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-97
EDTA plasma (n=4)9591-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.588 2.542 2.565 2.416
25001.788 1.745 1.767 1.618
12501.079 1.066 1.073 0.924
6250.698 0.701 0.700 0.551
312.50.443 0.434 0.439 0.290
1560.298 0.278 0.288 0.139
780.222 0.230 0.226 0.077
00.150 0.148 0.149  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Gene References into Functions
  1. tumor endothelial cells heterogeneity is regulated by SPARCL1, which promotes the cell quiescence and vessel homeostasis contributing to the favorable prognoses associated with Th1-tumor microenvironments colorectal carcinomas. PMID: 27721236
  2. Study shows that astrocyte-secreted hevin is a trans-synaptic linker that bridges presynaptic NRX1alpha with postsynaptic NL1B. This way, hevin organizes both pre- and postsynaptic specializations and aligns them across the synapse. PMID: 26771491
  3. Hevin is transiently expressed in the IrrPTK-injured corneas and loss of hevin predisposes them to aberrant wound healing. PMID: 24303054
  4. SC1 and SPARC may share significant homology but are likely to have distinct but complementary roles in central nervous system development. PMID: 22173850
  5. This study presented evidence that combined NL1 and SC1 overexpression triggers excitotoxic neurodegeneration through SC1 signaling at synaptic connections initiated by NL1. PMID: 22542674
  6. In gliomas implanted into murine brain, a SLF was associated with SPARC in the neovasculature but not with hevin, the latter prominent in the astrocytes encompassed by infiltrating tumor PMID: 21688302
  7. These results identify hevin as a positive and SPARC as a negative regulator of synapse formation. PMID: 21788491
  8. Hevin does not appear to be critical to regulating intraocular pressure. Hevin is expressed in trabecular meshwork but, in contrast to SPARC, does not appear to be regulated by TGF-beta2. PMID: 21220554
  9. proteolysis of hevin by ADAMTS4 in the mouse cerebellum is important for the normal development of this tissue PMID: 20018883
  10. determination of collagen I binding and examination of expression in a variety of tissues PMID: 12538579
  11. This hematopoietic stem cell enhancer ia bound by transription factors in stem cells and in hemangioblasts. PMID: 17962413
  12. Data show that Sparcl1 in the locus Rgcs1 region is highly expressed in the ganglion cell layer and optic nerve head. PMID: 18671875

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families SPARC family
Tissue Specificity Highest expression in brain. Moderate levels in heart, adrenal gland, epididymis and lung. Low levels in kidney, eye, liver, spleen, submandibular gland and testis.
Database Links

KEGG: mmu:13602

STRING: 10090.ENSMUSP00000031249

UniGene: Mm.29027

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