Mouse Tetranectin(CLEC3B) ELISA kit

Code CSB-EL005531MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse CLEC3B ELISA Kit was designed for the quantitative measurement of Mouse CLEC3B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Target Name C-type lectin domain family 3, member B
Alternative Names Clec3b ELISA Kit; Tna ELISA Kit; Tetranectin ELISA Kit; TN ELISA Kit; C-type lectin domain family 3 member B ELISA Kit; Plasminogen kringle 4-binding protein ELISA Kit
Abbreviation CLEC3B
Uniprot No. P43025
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLEC3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 104    
Range % 100-112    
1:200 Average % 90    
Range % 86-94    
1:400 Average % 86    
Range % 82-90    
1:800 Average % 93    
Range % 89-98    
Recovery
The recovery of mouse CLEC3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 88 84-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
100 2.221 2.380 2.301 2.162    
50 1.975 2.014 1.995 1.856    
25 1.616 1.528 1.572 1.433    
12.5 1.004 0.979 0.992 0.853    
6.25 0.634 0.652 0.643 0.504    
3.12 0.392 0.407 0.400 0.261    
1.56 0.250 0.243 0.247 0.108    
0 0.142 0.135 0.139      
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
Subcellular Location Secreted.
Tissue Specificity Highest expression in lung and skeletal muscle.
Database Links

STRING: 10090.ENSMUSP00000026890

UniGene: Mm.34588

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