Mouse Thyroid-Peroxidase,TPO ELISA Kit

Instructions
Code CSB-E08353m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name thyroid peroxidase
Alternative Names Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
Abbreviation TPO
Uniprot No. P35419
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 2.5 ng/mL-40 ng/mL
Sensitivity 2.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 90    
Range % 86-103    
1:2 Average % 89    
Range % 82-97    
1:4 Average % 95    
Range % 87-100    
1:8 Average % 90    
Range % 83-99    
Recovery
The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 94 87-102    
EDTA plasma (n=4) 95 90-103    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
40 0.272 0.263 0.268      
20 0.525 0.530 0.528      
10 0.894 0.874 0.884      
5 1.334 1.377 1.356      
2.5 2.554 2.528 2.541      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
Gene References into Functions
  1. Lnk Deficiency Leads to TPO-Mediated Osteoclastogenesis and Increased Bone Mass Phenotype PMID: 28067429
  2. Hearing impairment in hypothyroid dwarf mice caused by mutations of the thyroid peroxidase gene. PMID: 24297261
  3. A novel hypothyroid dwarfism is due to the missense mutation Arg479Cys of the thyroid peroxidase gene in the mouse. PMID: 16762971
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Peroxidase family, XPO subfamily
Database Links

KEGG: mmu:22018

STRING: 10090.ENSMUSP00000021005

UniGene: Mm.4991

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