Mouse Vitamin K-dependent protein S(PROS1) ELISA kit

Product Details

Customer Reviews and Q&A

Target Background

Product Details

Target Name

protein S (alpha)

Alternative Names

Pros1 ELISA Kit; ProsVitamin K-dependent protein S ELISA Kit

Abbreviation

PROS1

Uniprot No.

Q08761

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25 ng/mL-400 ng/mL

Sensitivity

1.56 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
	Range %	88-96
1:400	Average %	87
	Range %	84-90
1:800	Average %	95
	Range %	91-99
1:1600	Average %	97
	Range %	92-102

Recovery

The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
400	0.080	0.083	0.082
200	0.117	0.119	0.118
100	0.177	0.172	0.175
50	0.276	0.296	0.286
25	0.504	0.490	0.497
12.5	0.923	0.931	0.927
6.25	1.461	1.496	1.479
0	2.539	2.587	2.563

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse PROS1 ELISA Kit was designed for the quantitative measurement of Mouse PROS1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

Target Background

Function
(From Uniprot)

Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.

Gene References into Functions

An inhibitory role for PROS1 on BM-derived LCs. Collectively. PMID: 29871951
This study identifies a duple role for PROS1 in stem-cell quiescence and as a pro-neurogenic factor, and highlights a unique segregation of increased stem cell proliferation from enhanced neuronal differentiation. PMID: 27753164
Mice overexpressing protein S showed significant improvements in blood glucose level, glucose tolerance, insulin sensitivity, and insulin secretion compared with wild-type counterparts. diabetic protein S transgenic mice developed significantly less severe diabetic glomerulosclerosis than controls. PMID: 27207541
results support a causal relationship between the PS-K196E mutation and venous thrombosis PMID: 26251307
By revealing that neural stem-like cells act within the SVZ neurogenic niche as phagocytes and that the ProS/MerTK path represents an endogenous regulatory mechanism for SVZ cell phagocytic activity PMID: 25308179
Data indicate that activated T cells express Pros1. PMID: 23850380
Results demonstrate that Protein S is a Mer ligand, and is active in Mer-driven phagocytosis in the retina. PMID: 23259948
A self-regulatory mechanism of Toll-like receptor signalling through the suppression of Gas6 and ProS expression is described. PMID: 22043818
Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor1. PMID: 20348395
Data show that the spectrum of bioactivities of recombinant murine protein S with mouse plasma and smooth muscle cells is similar to that of human protein S. PMID: 19815836
results demonstrate that ProS is a pleiotropic anticoagulant with activated Protein C-independent activities and highlight new roles for ProS in vascular development and homeostasis PMID: 19729839
Pregnancy causes a decrease in APC resistance in mice, which can be explained by the elevation of protein S levels and increased TFPI activity in plasma. PMID: 19036061

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Subcellular Location

Secreted.

Tissue Specificity

Plasma.

Database Links

KEGG: mmu:19128

STRING: 10090.ENSMUSP00000023629

UniGene: Mm.127156

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Code	CSB-EL018754MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.