Mouse WAP four-disulfide core domain protein 2(WFDC2) ELISA kit

Code CSB-EL026092MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
WAP four-disulfide core domain 2
Alternative Names
Wfdc2 ELISA Kit; He4 ELISA Kit; WAP four-disulfide core domain protein 2 ELISA Kit; WAP domain-containing protein HE4 ELISA Kit
Abbreviation
WFDC2
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WFDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %81-95
1:2Average %96
Range %90-102
1:4Average %97
Range %92-105
1:8Average %95
Range %89-98
Recovery
The recovery of mouse WFDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9286-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.045 2.144 2.095 1.943
20001.624 1.743 1.684 1.532
10001.053 1.072 1.063 0.911
5000.673 0.663 0.668 0.516
2500.342 0.351 0.347 0.195
1250.274 0.283 0.279 0.127
62.50.229 0.235 0.232 0.080
00.152 0.151 0.152  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse WFDC2 ELISA Kit was designed for the quantitative measurement of Mouse WFDC2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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 Q&A
Q:

I have a few questions on product CSB-EL026092MO :
1. Which is this standard in this kit, natural material or recombinant?
If recombinant, please let us know expression method(E.coli etc.).
2. Is the standard or antibody, including any additive agent(antiseptic
agent or preserving agent)? If so, please let us know composition and
concentration.
3. Is this product including Stop solution? If so, please tell us
composition and concentration for stop solution.
4. Please let us know whether this kit include any hazardous
substances(azide, thimerosal, 2-ME, etc). If so, please let us know
composition and concentration.

A:
Thanks for your inquiry!
Pls check our answer:
1, Standard of CSB-EL026092MO is NS0-derived recombinant protein
2,Preservative of this kit is proclin 300(1:3000)
3, Stop solution: 4N sulfuric acid
4, There is no hazardous substances (azide, thimerosal, 2-ME, etc) included in the kit.

Target Background

Function
(From Uniprot)
Broad range protease inhibitor.
Gene References into Functions
  1. Causal testing implicates Wfdc2 and Hdac3 as novel genes affecting high density lipoprotein levels PMID: 21807117
Subcellular Location
Secreted.
Database Links
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