Mouse anti-Smith antibody ELISA Kit

Anti-Smith antibodies are autoantibodies that target the Smith antigen, a core component of small nuclear ribonucleoproteins involved in pre-mRNA splicing. These antibodies serve as highly specific markers for systemic lupus erythematosus and are included in classification criteria for this autoimmune disease. Anti-Smith antibodies correlate with more severe disease manifestations and provide valuable diagnostic and prognostic information in clinical and research settings.

The Mouse anti-Smith antibody ELISA Kit (CSB-E15976m) uses a quantitative sandwich measurement principle to detect anti-Smith antibodies in mouse serum and plasma samples. This assay offers a detection range of 0.625 ng/mL to 40 ng/mL with a sensitivity of 0.156 ng/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in autoimmune disease research to quantify anti-Smith antibodies in mouse serum samples. The applications focus on measuring autoantibody levels as part of comprehensive autoimmune profiling studies that examine multiple antibody markers simultaneously.

• Autoimmune disease research: Quantification of anti-Smith antibodies in mouse serum as part of autoantibody profiling studies
• Systemic lupus erythematosus models: Evaluation of autoantibody production in experimental mouse models alongside other lupus-related markers
• Biomarker studies: Measurement of anti-Smith antibody levels in conjunction with other autoimmune markers for comprehensive immune system evaluation