Mouse anti-double stranded DNA antibody (IgG) ELISA Kit

Code CSB-E11194m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Abbreviation dsDNA Ab (IgG)
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Capture ELISA
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-double stranded DNA antibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 82-98  
1:2 Average % 96  
Range % 90-101  
1:4 Average % 96  
Range % 92-101  
1:8 Average % 87  
Range % 81-96  
The recovery of mouse anti-double stranded DNA antibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 85-98  
EDTA plasma (n=4) 95 92-101  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.807 2.912 2.860 2.663  
10 2.315 2.456 2.386 2.189  
5 1.568 1.631 1.600 1.403  
2.5 0.835 0.875 0.855 0.658  
1.25 0.526 0.524 0.525 0.328  
0.625 0.353 0.329 0.341 0.144  
0.312 0.254 0.243 0.249 0.052  
0 0.193 0.200 0.197    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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1. What is the standard in the kit?
If this is a recombinant protein, what is the expression method?
Is it expressed in E.coli?
2. What is the stop solution? We need to know the material and quantity.

Thanks for your inquiry.
The standard of this kit CSB-E11194m is the native sample crude extract(recombinant protein)
Stop solution: 4N sulfuric acid
The detailed kit components and quantity, pls check the matarials provided part on page 4 from the manual.I will send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.


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