Mouse calcitonin gene related peptide (CGRP) ELISA kit

Code CSB-EQ027706MO
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name calcitonin gene related peptide (CGRP)
Alternative Names Beta type CGRP、Calcitonin gene-related peptide II、CGRPII、Calcitonin gene-related peptide 2、CALCB
Abbreviation CGRP
Uniprot No. Q99MP3
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %86-98
1:2Average %92
Range %88-97
1:4Average %90
Range %85-96
1:8Average %94
Range %88-99
Recovery
The recovery of mouse CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-97
EDTA plasma (n=4)9691-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
\\\"\\\"
pg/ml OD1 OD2 Average Corrected  
1000 2.342 2.321 2.332 2.178  
500 1.772 1.760 1.766 1.612  
250 1.250 1.241 1.246 1.092  
125 0.799 0.789 0.794 0.640  
62.5 0.499 0.487 0.493 0.339  
31.25 0.364 0.341 0.353 0.199  
15.6 0.253 0.244 0.249 0.095  
0 0.156 0.152 0.154    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
Subcellular Location Secreted.
Protein Families Calcitonin family
Tissue Specificity Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.
Database Links

STRING: 10090.ENSMUSP00000032902

UniGene: Mm.273838

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