Mouse cross linked C-telopeptide of type I collagen (CTX-Ⅰ) ELISA Kit

Instructions
Code CSB-E12782m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name cross linked C-telopeptide of type I collagen (CTX-Ⅰ)
Alternative Names cross linked C-telopeptide of type 1 collagen (CTX-1),cross linked C-telopeptide of type I collagen (CTX-I)
Abbreviation CTX-Ⅰ
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.075 ng/mL-15 ng/mL
Sensitivity 0.04 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 92-99  
1:2 Average % 95  
Range % 89-102  
1:4 Average % 94  
Range % 85-103  
1:8 Average % 96  
Range % 90-102  
Recovery
The recovery of mouse CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 91-105  
EDTA plasma (n=4) 96 92-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
15 0.185 0.174 0.180    
3.75 0.326 0.332 0.329    
1.2 0.526 0.543 0.535    
0.3 0.824 0.854 0.839    
0.075 1.329 1.323 1.326    
0 1.967 2.002 1.985    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

What's the antibody information of this kit?

A:
Thanks for your inquiry. Pls check our information: CSB-E12782m: Coated anti. Goat against rabbit secondary antibody; Specific anti: Rabbit polyclonal antibody
Coated anti. Goat against rabbit secondary antibody; Antigen Specific anti: Rabbit polyclonal antibody. Pls let me know if you have any further questions. Thank you.

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