Mouse diamine oxidase,DAO ELISA Kit

Code CSB-E10090m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name amiloride binding protein 1 (amine oxidase (copper-containing))
Alternative Names Aoc1 ELISA kit; Abp1Amiloride-sensitive amine oxidase [copper-containing] ELISA kit; DAO ELISA kit; Diamine oxidase ELISA kit; EC ELISA kit; Amiloride-binding protein 1 ELISA kit; Amine oxidase copper domain-containing protein 1 ELISA kit; Histaminase ELISA kit
Abbreviation DAO
Uniprot No. Q8JZQ5
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 mU/mL-1000 mU/mL
Sensitivity 3.9 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %84
Range %81-87
1:2Average %95
Range %91-99
1:4Average %91
Range %88-96
1:8Average %96
Range %93-99
The recovery of mouse DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 109104-112
EDTA plasma (n=4)8581-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.879 2.752 2.816 2.607
5002.301 2.216 2.259 2.050
2501.584 1.560 1.572 1.363
1250.945 0.932 0.939 0.730
62.50.655 0.672 0.664 0.455
31.20.532 0.542 0.537 0.328
15.60.412 0.407 0.410 0.201
00.211 0.206 0.209
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis.
Subcellular Location Secreted, extracellular space
Protein Families Copper/topaquinone oxidase family
Database Links

STRING: 10090.ENSMUSP00000031835

UniGene: Mm.213898

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