Mouse fibrin ELISA kit

Code CSB-EQ027562MO
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
fibrin
Abbreviation
fibrin
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse fibrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %93
Range %87-103
1:200Average %96
Range %86-102
1:400Average %92
Range %84-100
1:800Average %94
Range %85-100
Recovery
The recovery of mouse fibrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9789-101
EDTA plasma (n=4)9589-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.766 2.684 2.725 2.603
5001.838 1.902 1.870 1.748
2501.123 1.085 1.104 0.982
1250.665 0.636 0.651 0.529
62.50.384 0.365 0.375 0.253
31.250.283 0.301 0.292 0.170
15.60.199 0.205 0.202 0.080
00.124 0.120 0.122
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Mouse fibrin ELISA Kit was designed for the quantitative measurement of Mouse fibrin protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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