Mouse glial cell line-derived neurotrophic factor,GDNF ELISA KIT

Code CSB-E07341m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glial cell derived neurotrophic factor
Alternative Names GdnfGlial cell line-derived neurotrophic factor ELISA Kit; mGDNF ELISA Kit; Astrocyte-derived trophic factor ELISA Kit; ATF ELISA Kit
Abbreviation GDNF
Uniprot No. P48540
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.9 pg/mL-60 pg/mL
Sensitivity 0.23 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 91-101  
1:2 Average % 102  
Range % 99-104  
1:4 Average % 89  
Range % 86-92  
1:8 Average % 94  
Range % 92-96  
The recovery of mouse GDNFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 94-100  
EDTA plasma (n=4) 95 92-108  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
60 1.988 2.152 2.070 1.910  
30 1.354 1.368 1.361 1.201  
15 0.806 0.822 0.814 0.654  
7.5 0.548 0.534 0.541 0.381  
3.75 0.372 0.383 0.378 0.218  
1.8 0.274 0.295 0.285 0.125  
0.9 0.211 0.224 0.218 0.058  
0 0.161 0.159 0.160    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Gene References into Functions
  1. lack of Kir6.2 promoted neuronal differentiation via inhibiting the downregulation of glia cell line-derived neurotrophic factor (GDNF), which negatively related to the level of microRNA-133b. PMID: 29564810
  2. These results imply an important role of GDNF in restoring restrictive blood-nerve barrier function in vivo PMID: 29913111
  3. These results suggest that chronic overexpression of GDNF in brain astrocytes exerts an opposing influence on nigrostriatal dopamine metabolism and neurotransmission. PMID: 28645787
  4. GDNF promotes self-renewal by blocking differentiation and not by promoting proliferation. PMID: 29440301
  5. Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf. PMID: 29183737
  6. Our study provides, for the first time, a global analysis of phosphorylation events in spermatogonial progenitor cells (SPCs) in response to GDNF, and we have identified activation of mTORC1 signaling through ERK kinase-mediated phosphorylation of multiple sites of raptor protein as an important pathway for SPC proliferation. PMID: 28408662
  7. Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity. PMID: 27119228
  8. Six2 mediates the protective effects of GDNF on damaged DA neurons by regulating Smurf1 expression. PMID: 27148690
  9. Ret is essential to mediate GDNF's neuroprotective and neuroregenerative effect in a Parkinson disease mouse model. PMID: 27607574
  10. identified IFNg, Neurturin (Nrtn), and glial-derived neurotrophic factor (GDNF) as ligands with unexpected roles in promoting neurogenic differentiation of Neural Precursor Cells in vivo. PMID: 27545711
  11. Using an organ culture system for prostate development and Ret mutant mice, we demonstrate that RET-mediated GDNF signaling in UGS increases proliferation of mesenchyme cells and suppresses androgen-induced proliferation and differentiation of prostate epithelial cells, inhibiting prostate development. PMID: 28506996
  12. The GDNF-GFRalpha1 complex is essential for proper hippocampal circuit development. PMID: 27707798
  13. In SOD1(G93A) spinal cords, we verified a strict correlation in the expression of the TNFalpha, TNFR1 and GDNF triad at different stages of disease progression. Yet, ablation of TNFR1 completely abolished GDNF rises in both SOD1(G93A) astrocytes and spinal cords, a condition that accelerated motor neuron degeneration and disease progression PMID: 27288458
  14. GDNF signals were able to induce the stratified aggregate formation of GFRalpha1-positive undifferentiated spermatogonia PMID: 27255992
  15. The Gdnf cKO males sired up to two litters but became infertile due to collapse of spermatogenesis and loss of undifferentiated spermatogonia PMID: 26831079
  16. Our results reveal the role of GDNF in nigrostriatal dopamine system postnatal development and adult function, and highlight the importance of correct spatial expression of GDNF PMID: 26681446
  17. Mice overexpressing GDNF had significantly reduced P62 protein levels suggestive of accelerated autophagy. They also had reduced PPAR-gamma and CD36 gene expression and protein levels, and lower expression of mRNA coding for enzymes involved lipogenesis. PMID: 26564715
  18. This study demonstrated that decrease in mRNA level of GDNF in brain in microgravity. PMID: 25944479
  19. GDNF signaling in the urogenital sinus increases proliferation. PMID: 25549043
  20. Results show that in the inflamed intestine, smooth muscle proliferation supports the enteric nervous system , and thus its own re-innervation, by expression of GDNF PMID: 25655216
  21. Parkin and the RET signaling cascade converge to control mitochondrial integrity and thereby properly maintain substantia nigra pars compacta dopaminergic neurons and their innervation in the striatum. PMID: 25822020
  22. expression induced by testosterone in peritubular myoid cells PMID: 25181385
  23. These data suggest that there are significant changes in the responses to GDNF as spermatogonial stem cells give rise to progenitor spermatogonia. PMID: 25165119
  24. The transcriptional start site and the promoter activity of the 5'-flanking region of GDNF have been identified. PMID: 25025809
  25. GDNF expression was upregulated upon radiation therapy in submandibular glands. PMID: 25036711
  26. This hypothesis offers a dramatically different explanation for the etiology of sporadic Parkinson's disease as a manifestation of acquired resistance to GDNF. PMID: 25448159
  27. Study highlights role of GDNF on striatal organization, both on DARPP-32-positive neurons and tyrosine hydroxylase (TH)-positive innervation; and importance of GDNF expression in striatum for TH-positive neuron viability when connected to striatum PMID: 24726488
  28. A role is found for proGDNF signaling in brains from aging and Parkinson's disease animal models. PMID: 23934644
  29. The data suggest that genetically defined cross-talk between neurotrophic factors and the brain serotonin system underlies the variability in behavioral response to GDNF. PMID: 24105724
  30. These findings suggest that pleiotropic function of GDNF is at least in part governed by modulating levels of intracellular activation of GDNF downstream pathways PMID: 24107967
  31. we show that GDNF is capable of promoting survival of 30% sympathetic precursors dissociated at embryonic day 13 PMID: 23426908
  32. GDNF is required for neural colonization of the pancreas. PMID: 23903190
  33. studies further support that long-term changes related to the dysfunction of the nigrostriatal pathway are influenced by GDNF expression PMID: 23290934
  34. injection of GL261 mouse glioma cells with GDNF knockdown by shRNA into mouse brains resulted in reduced tumor expansion and improved survival as compared to injection of control cells. PMID: 23344256
  35. SorLA acts as sorting receptor for the GDNF/GFRalpha1 complex, directing it from the cell surface to endosomes. PMID: 23333276
  36. Neuroprotective effects of carotid body transplantation in an MPTP model of Parkinson disease depends on the level of GDNF expression. PMID: 22743091
  37. spermatogonial stem cell chemotactic factor in vitro and in vivo PMID: 23040482
  38. analysis of neuroprotection by GDNF in the ischemic brain [review] PMID: 22670840
  39. This tsudy demonistrated the role of GDNF for embryonic Schwann cells migration. PMID: 22863354
  40. Targeted overexpression of GDNF in the muscles of Myo-GDNF mice did not improve motor recovery after botulinum toxin type A chemodenervation. PMID: 22473739
  41. This study identifies a regulator of midline crossing and reveals interplays between Semaphorin and gdnf signaling during axon guidance. PMID: 22998873
  42. demonstrate functionally antagonistic eicosanoid receptor subtype regulation of innate immunity-induced astrocyte GDNF expression PMID: 22499581
  43. GDNF, besides its crucial role in the self-renewal of stem cells also functions in the differentiation of chained undifferentiated spermatogonia. PMID: 22143971
  44. These results provide the first direct proof that GDNF acutely regulates the number of spermatogonial stem cells in the normal adult testis. PMID: 22232066
  45. A AT rich region in the GDNF 3' UTR have a suppressive role its gene expression. PMID: 22248285
  46. we suggest that NANOS2 acts downstream of GDNF signaling to maintain undifferentiated state of spermatogonial stem cells. PMID: 22102605
  47. These data provide direct evidence of regionally defined patch-like GDNF-positive signal. PMID: 22174794
  48. ETV5 was found to be a critical downstream regulator of GDNF signaling that mediated the expression of several known spermatogonial stem cell self-renewal related genes, including Bcl6b and LIM homeobox. PMID: 21816850
  49. Addition of motor neurons to skeletal muscle cells in vitro reduces the secretion of GDNF by skeletal muscle. PMID: 21420941
  50. In this article showed that GDNF production in mouse striatum develops in early postnatal life and is essentially confined to neurons. PMID: 22262884

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Subcellular Location Secreted
Protein Families TGF-beta family, GDNF subfamily
Tissue Specificity Expressed in both the central nervous system (CNS) and in non-CNS tissues. Expressed in a highly dynamic pattern in the anterior neuroectoderm during the early stages of neurogenesis between E7.5 and E10.5. Beginning at E10.5, expression begins in mesench
Database Links

KEGG: mmu:14573

STRING: 10090.ENSMUSP00000022744

UniGene: Mm.4679


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