Mouse lipoprotein lipase,LPL ELISA Kit

Code CSB-E08495m
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
lipoprotein lipase
Alternative Names
Lpl ELISA Kit; Lipoprotein lipase ELISA Kit; LPL ELISA Kit; EC ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
0.156 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 87-93  
1:2 Average % 92  
Range % 88-95  
1:4 Average % 94  
Range % 90-97  
1:8 Average % 100  
Range % 95-103  
The recovery of mouse LPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 91-97  
EDTA plasma (n=4) 95 92-98  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.409 2.348 2.379 2.233  
20 1.788 1.767 1.778 1.632  
10 1.102 1.088 1.095 0.949  
5 0.691 0.688 0.690 0.544  
2.5 0.444 0.434 0.439 0.293  
1.25 0.301 0.308 0.305 0.159  
0.625 0.232 0.242 0.237 0.091  
0 0.144 0.148 0.146    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse LPL ELISA Kit was designed for the quantitative measurement of Mouse LPL protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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1) Concentration of sodium azide or thimerosal and 2-mercaptoethanol(or other toxic substances) if it is contained in the product.
2) Please let us know whether the CSB-E08495m was produced with Baculovirus to
follow Cartagena protocol regulation.

Thanks for your inquiry.
The stop solution is 4N sulfuric acid.
There is no sodium azide or thimerosal and 2-mercaptoethanol(or other toxic substances) in the kit.
The standard is recombinant protein expressed in the eukaryotic system. There is no Baculovirus derived component.

Target Background

(From Uniprot)
Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.
Gene References into Functions
  1. LPL-mediated release of essential fatty acid DHA regulates hematopoietic stem progenitor cell expansion and definitive hematopoiesis PMID: 29615667
  2. the negatively charged IDR of GPIHBP1 traverses a vast space, facilitating capture of LPL by capillary endothelial cells and simultaneously contributing to GPIHBP1's ability to preserve LPL structure and activity. PMID: 29899144
  3. LPL in the hypothalamus is an important regulator of body weight and glucose homeostasis PMID: 28456865
  4. These results identify LPL as an important regulator of fatty acid transport to skeletal compartments and demonstrate an intricate functional link between systemic and skeletal fatty acid and glucose metabolism. PMID: 28608812
  5. mutation of a conserved cysteine in GPIHBP1 abolishes the ability of GPIHBP1 to bind LPL PMID: 28476858
  6. The data suggests that ANGPTL3 is part of the machinery causing dyslipidemia majorily via LPL inhibition in mastitis mice. PMID: 29104012
  7. Using in vitro ketosis model by glucose starvation, studied inhibition of ketosis by momilactone B. Found momilactone B could regulate the angiopoietin-like-3 (ANGPTL3)-lipoprotein lipase (LPL)pathway, and suppressed the expression of HMGCS2 through the increased expression of STAT5b. PMID: 27874312
  8. physiological changes in adipose tissue ANGPTL4 expression during fasting and cold resulted in inverse changes in the amount of mature-glycosylated LPL in wild-type mice, but not Angptl4(-/-) mice. We conclude that ANGPTL4 promotes loss of intracellular LPL by stimulating LPL degradation after LPL processing in the endoplasmic reticulum (ER). PMID: 27034464
  9. LPL moved quickly from heparan sulfate proteoglycans (HSPGs) on adipocytes to GPIHBP1-coated beads, thereby depleting LPL stores on the surface of adipocytes. We conclude that HSPG-bound LPL in the interstitial spaces of tissues is mobile, allowing the LPL to move to GPIHBP1 on endothelial cells PMID: 27811232
  10. our study reveals that hepatic LPL is involved in the regulation of plasma LPL activity and lipid homeostasis. PMID: 27234787
  11. The induction of LPL activity by fasting in core transgenic mice activated PPARalpha downstream target genes that are involved in fatty acid beta-oxidation. PMID: 27665576
  12. This study shows that TNF-alpha, by a Foxo1 dependent pathway, increases the transcription of ANGPTL4 which is secreted by the cells and causes inactivation of LPL. PMID: 28215713
  13. Our findings suggest that neuronal LPL is involved in the regulation of body weight and composition in response to either the change in quantity (HF feeding) or quality (n-3 PUFA-enriched) of dietary fat PMID: 27282869
  14. An LPL structural model suggests that the LPL S447X truncation exposes residues implicated in LPL binding to lipoprotein binding uptake receptors, such as GPIHBP1. PMID: 27984852
  15. feeding induces lipasin, activating the lipasin-Angptl3 pathway, which inhibits LPL in cardiac and skeletal muscles to direct circulating TAG to WAT for storage PMID: 26687026
  16. MiR-590 agomir down-regulates LPL mRNA and protein expression in a mouse model of atherosclerosis. PMID: 26397958
  17. Deficiency of Lipoprotein Lipase in Neurons Decreases AMPA Receptor Phosphorylation and Leads to Neurobehavioral Abnormalities in Mice PMID: 26263173
  18. Systemic LPL deletion results in impaired glucose tolerance, whole-body and tissue-specific insulin resistance, which is associated with tissue lipid deposition in various insulin target tissues PMID: 25635326
  19. Results indicated that aggregation of alpha-syn and reduction of UCHL1 expression in LPL-deficient mice may affect synaptic function. PMID: 25595992
  20. the amount of LPL expressed in muscle and heart governed both the binding of chylomicron particles and the assimilation of chylomicron lipids in the tissue. PMID: 25589507
  21. Maternal overnutrition induces LPL expression in trophoblasts by reducing the inhibitory effect of SIRT1 on PPARgamma. PMID: 25948680
  22. Lipoprotein lipase is an important modulator of lipid uptake and storage in hypothalamic neurons. PMID: 26265042
  23. Results suggest that impaired synaptic vesicle recycling results from deficient docosahexaenoic acid and arachidonic acid and contributes to the presynaptic dysfunction and plasticity impairment in LPL-deficient neurons PMID: 25194787
  24. Adipocyte-specific Sel1L-deficient (AKO) mice are resistant to diet-induced obesity. Sel1L stabilizes and prevents LPL dimers from aggregation in the endoplasmic reticulum. PMID: 25066055
  25. This study showed that phloridzin improved plasma lipoprotein lipase activity via a decrease of ANGPTL4 mRNA expression and an increase of AMP-activated protein kinase phosphorylation. PMID: 24932810
  26. TRL margination depends on LPL bound to GPIHBP1. PMID: 24726386
  27. LpL hydrolysis of circulating lipoproteins is required for the accumulation of lipids in the heart of fasting mice. PMID: 24493834
  28. The expression levels of miR-27a and miR-29a inversely correlate with the mRNA levels of lipoprotein lipase and its key transcriptional regulator peroxisome proliferator-activated receptor gamma during 3T3-L1 adipocyte differentiation. PMID: 24457907
  29. Leu452His mutation in lipoprotein lipase gene transfer associated with hypertriglyceridemia in mice in vivo. PMID: 24086538
  30. PPARgamma1 was intimately involved in LPL gene expression in skeletal muscle and the AMPK-PPARgamma1 pathway may play a role in exercise-induced LPL expression. PMID: 24644240
  31. Activated cyclin-dependent kinase 5 promotes microglial phagocytosis of fibrillar beta-amyloid by up-regulating lipoprotein lipase expression. PMID: 23816988
  32. inactivation of LPL by Angptl4 appears to occur after both proteins have traveled along the secretory pathway and arrived at the cell surface. PMID: 24220340
  33. The present study showed that miR-467b protects apoE(-/-) mice from atherosclerosis by reducing lipid accumulation and inflammatory cytokine secretion via downregulation of LPL expression. PMID: 24309104
  34. Lipoprotein lipase activity decreases in adipose tissue during fasting. PMID: 23176178
  35. Macrophage LpL plays an important role in the development of atherosclerosis but not adiposity. PMID: 23378601
  36. LpL has a role as the "gatekeeper" for tissue lipid distribution and its deficiency more profoundly affects brown than white fat biology PMID: 23542081
  37. Neither a high fat diet nor fasting/re-feeding markedly altered the distribution pattern of LPL or GPIHBP1 in mouse pancreas. PMID: 23186339
  38. Findings indicate that miR-467b may regulate lipid accumulation and proinflammatory cytokine secretion in oxLDL-stimulated macrophages by targeting the LPL gene. PMID: 22963823
  39. ABCG1 controls LPL activity and promotes lipid accumulation in human macrophages in the presence of triglyceride-rich lipoproteins. PMID: 22772754
  40. variation in Lmf1 expression is a posttranslational determinant of LPL activity. PMID: 22345169
  41. lipoprotein retention in Bruch's membrane is mediated by lipoprotein lipase PMID: 21801873
  42. Hematopoietic cell-derived LPL could efficiently ameliorate severe hypertriglyceridemia and hypo-alpha-cholesterolemia at the compensation of increased triglyceride content of liver PMID: 21980507
  43. These results suggest that downregulation of miR-467b is involved in the development of hepatic steatosis by modulating the expression of its target, LPL. PMID: 21986524
  44. Uptake of dietary retinoids at the maternal-fetal barrier: in vivo evidence for the role of lipoprotein lipase and alternative pathways. PMID: 21795711
  45. LPL gene expression appears to be under dietary control: supplementation with cyanidin-3-O-beta-glucoside appears to up-regulate LPL in plasma and skeletal muscle but down-regulate LPL in visceral adipose tissue in the KK-Ay mouse model of diabetes PMID: 21360538
  46. the cleavage of ANGPTL4 by these PCs modulates its inhibitory effect on LPL activity. PMID: 21398697
  47. LPL is a novel Abeta-binding protein promoting cellular uptake and subsequent degradation of Abeta. PMID: 21177248
  48. Data show that apoC-II and LPL mRNAs correlate temporally and geographically with surfactant lipid synthesis in preparation for birth and suggest that fatty acid recruitment from the circulation by apoC-II-activated LPL is modulated by apoC-II secretion. PMID: 21059267
  49. PPAR-alpha response is generated by unbound fatty acids released locally by lipase activity and not by circulating plasma fatty acids. PMID: 20421589
  50. Results show that cotransfection of LPL with wild-type Lmf1 restores its ability to support normal lipase maturation. PMID: 19471043

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Subcellular Location
Cell membrane; Peripheral membrane protein; Extracellular side. Secreted. Secreted, extracellular space, extracellular matrix.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Detected in white and brown adipose tissue and heart muscle, especially at the lumenal surface of capillaries. Detected on capillary endothelium in the lactating mammary gland. Detected in blood plasma (at protein level). Expressed in liver, epididymal fa
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