Thanks for your inquiry. Our replies are as below.
Recombinant Mouse Lipoprotein lipase(Lpl)
CSB-YP013065MO >> Yeast
CSB-EP013065MO >> E.coli
CSB-BP013065MO >> Baculovirus
CSB-MP013065MO >> Mammalian cell
Expression Region: 28-474aa; Full length of the mature protein.
Tag information:EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged; YP: N-terminal 6xHis-tagged.
We have successfully developed the CSB-EP013065MO, but you mentioned that you are especially interested in MP protein, sorry, we haven't expressed this MP protein before, also have never tried to remove the tag.
In theory, as we already successfully expressed the EP protein, the MP is more likely to be successfully expressed, regarding the tag removal at both of N- and C- terminus,
Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order. We can try enzyme digestion, but we can't guarantee 100% success.
The overall success rate of enzyme digestion data analysis is 75%-86%.
* Not all protein tags can be removed as some proteins will be very unstable after tag removal.
* If we succeed in removing the tag, we will charge for extra cost.
* If we fail in removing the tag, we won’t charge for any extra cost, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
* Generally, the delivery time will be extended for 3 days.
Regarding the biological activity, sorry, we haven't tested the biological activity of this protein before, probably you can measure the activity by yourself.
There are many kinds of activity detection kits on the market, in theory, there should be no problem in testing the activity.
But we should explain in advance that as we haven't tested the activity, so we can't 100% guarantee its biological activity.