Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit

Code CSB-E07409m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
matrix metallopeptidase 7 (matrilysin, uterine)
Alternative Names
Mmp7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
Abbreviation
MMP7
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %90-95
1:2Average %102
Range %98-104
1:4Average %92
Range %88-96
1:8Average %99
Range %95-102
Recovery
The recovery of mouse MMP7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9492-96
EDTA plasma (n=4)9692-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.180 2.088 2.134 1.981
10001.548 1.534 1.541 1.388
5000.882 0.899 0.891 0.738
2500.511 0.489 0.500 0.347
1250.338 0.331 0.335 0.182
62.50.256 0.248 0.252 0.099
31.250.202 0.211 0.207 0.054
00.154 0.152 0.153  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse MMP7 ELISA Kit was designed for the quantitative measurement of Mouse MMP7 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

Customer Reviews and Q&A

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 Q&A
Q:

 Does the kit contain human and/or animal by-products? What is the standard made of?

A:
Thanks for your inquiry. Standard is NS0-derived recombinant mouse FBLN1 protein.
The kit does not contain any human and/or animal by-products.

Target Background

Function
(From Uniprot)
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.; May play a role in tissue reorganization.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Database Links
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7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
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