Mouse matrix metalloproteinase 13,MMP-13 ELISA kit

Code CSB-E07413m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse MMP13 ELISA Kit was designed for the quantitative measurement of Mouse MMP13 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 23.438 pg/mL.

Target Name matrix metallopeptidase 13 (collagenase 3)
Alternative Names Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit
Abbreviation MMP13
Uniprot No. P33435
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.438 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %85-100
1:2Average %92
Range %90-100
1:4Average %96
Range %92-100
1:8Average %93
Range %82-98
Recovery
The recovery of mouse MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9281-98
EDTA plasma (n=4)9492-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
60002.720 2.642 2.681 2.453
30002.416 2.381 2.399 2.171
15002.112 2.043 2.078 1.850
7501.732 1.690 1.711 1.483
3751.337 1.244 1.291 1.063
187.50.914 0.876 0.895 0.667
93.750.534 0.492 0.513 0.285
00.237 0.219 0.228  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Gene References into Functions
  1. Overexpression of Dlx2 enhances early chondrocyte differentiation by increasing accumulation of Col2alpha1, type II collagen and aggrecan, but interferes later stages of chondrocyte differentiation through inhibiting MMP13 expression. PMID: 29787757
  2. Malignant extracellular vesicles carrying MMP1 mRNA facilitate peritoneal dissemination in ovarian cancer. PMID: 28262727
  3. Estrogen-related receptor gamma (ERRgamma) overexpression in chondrocytes directly upregulates matrix metalloproteinase (MMP)-3 and MMP13, which are known to play crucial roles in cartilage destruction in osteoarthritis (OA). PMID: 29247173
  4. MMP-2 promoted and MMP-13 disrupted vasculogenic mimicry formation in large cell lung cancer by cleaving laminin-5 to influence EGFR signal activation. PMID: 28766880
  5. The data demonstrate that MMP13 and CTGF play a crucial role in modulation of fibrogenic mediators while promoting hepatic fibrogenesis. PMID: 28782260
  6. CXCL10 has strong inhibitory effects on neovascularization, whereas MMP13 is required for neovascularization in C. albicans-infected corneas. PMID: 28623423
  7. that MMP-13 is a central protease in infarct development and cortical remodeling during post-stroke neurorepair PMID: 27001146
  8. the phenotype seen in the Hdac4(-/-) mice is partially derived from elevation in MMP-13 and may be due to a bone remodeling disorder caused by overexpression of this enzyme. PMID: 27320207
  9. data reveal a novel involvement of MMP-13 in regulating dendritic cell (DC) immunobiology through moderating MHC-I surface presentation, endocytosis and cytokine/chemokine secretion; furthermore, the reduced MHC-I surface presentation by DCs resulted in a poor CD8(+) T-cell response in vitro; MMP-13 might be a promising target for therapeutic intervention in inflammatory diseases PMID: 26921214
  10. The progressive process of articular cartilage degeneration was significantly delayed in the knee joints of Ddr2-deficient mice in comparison to their control littermates. Articular cartilage damage in the knee joints of the mice was associated with increased expression profiles of both Ddr2 and matrix metalloproteinase 13. PMID: 27640147
  11. Postnatal chondrocyte-specific deletion of Hdac3 with an inducible Col2a1-Cre caused premature production of pErk1/2 and Mmp13 in the growth plate. PMID: 27662443
  12. Mmp13 is selectively regulated of by 1,25-Dihydroxyvitamin D3, PTH, and Osterix through distal enhancers. PMID: 26348136
  13. Our study contributes to the understanding of the role of HIF1alpha in OA and highlights the HIF1alpha-beta-catenin interaction, thus providing new insights into the impact of hypoxia in articular cartilage. PMID: 27122313
  14. Matrix metalloproteinases (MMP) are effectors of hippocampal neuroplasticity in the adult central nervous system and that the MMP-1/protease-activated receptor-1 axis may play a role in neurogenesis following physiological and/or pathological stimuli. PMID: 26783471
  15. We reveal a novel role of AP-2epsilon in the regulation of gene expression in articular chondrocytes, as well as in osteoarthritis development, through modulation of Mmp13 expression and activity. PMID: 25964075
  16. B-16 melanoma cells with up-regulated MMP-13 expression promoted invasion of tumor cells through Matrigel compared with control cells transfected with empty vector. Knocked-down MMP-13 expression also decreased invading cell numbers. PMID: 25749207
  17. Data indicate that tamoxifen decreased collage type II COL2A1 by activating matrix metallopeptidases MMP-2/13 expression. PMID: 25703139
  18. This study demonstrates that stromal as well as tumor derived MMP13 contribute to tumor cell extravasation and establishment of metastases in the liver microenvironment. PMID: 25880591
  19. Elevated MMP13 activity may contribute to Pseudomonas aeruginosa keratitis through basement membrane degradation. PMID: 25589337
  20. Mmp13 gene is regulated via at least three specific distal enhancers that display independent activities yet are able to integrate response from multiple signaling pathways in a model of activation and suppression. PMID: 25773540
  21. SIRT1 activation inhibits PTH stimulation of Mmp13 expression, and this regulation is mediated by SIRT1 association with c-Jun at the AP-1 site of the Mmp13 promoter. PMID: 25631045
  22. Mmp13 is critical for Osteoarthritis progression PMID: 23298463
  23. the concomitant inhibition of MMP-9 and MMP-13 altered the immunogenic properties of melanoma, stimulated the lymphocytic infiltration of tumor nodules, and decreased the expression of oncomicroRNA miR-21 and miR-let-7b. PMID: 25868368
  24. there is a strong link between lower estrogen levels in old female mice, an elevated MMP-13 level, which results in a more effective MMP-13 inhibitor treatment in fibroblasts and SCC cells in vitro and in vivo PMID: 24676718
  25. MMP-13 deficiency acts to suppress the local inflammatory responses. Therefore, MMP-13 has a role in the pathogenesis of arthritis PMID: 24369907
  26. Knee loading reduces MMP13 activity and suppresses cartilage destruction. PMID: 24180431
  27. MMP-13 prevails over MMP-8 in collagen degradation in atheromata. PMID: 24723558
  28. Stromal matrix metalloprotease-13 knockout alters Collagen I structure at the tumor-host interface and increases lung metastasis of C57BL/6 syngeneic E0771 mammary tumor cells. PMID: 24010522
  29. ANKRD1, in association with factors such as nucleolin, represses MMP13 transcription. Ankrd1 deletion additionally relieved MMP10 transcriptional repression PMID: 24515436
  30. Relaxin and estrogen caused greater induction of MMP13 vs. MMP9 in temporomandibular joint. PMID: 24640575
  31. presence of MMP-13 seems to limit the overall extent of ECM deposition in lung fibrosis PMID: 24023851
  32. epithelial expression of MMP-1 inhibits mitochondrial function, increases HIF-1alpha expression, decreases reactive oxygen species production, and contributes to a proliferative, migratory, and anti-apoptotic AEC phenotype. PMID: 23902766
  33. Mmp13-dependent intestinal Tumor Necrosis Factor activation results in mucus depletion, increased intestinal inflammation, systemic inflammation and organ damage. PMID: 23723167
  34. MMP-13 has a primary role in myoblast migration that leads to secondary effects on differentiation, and is a new contributor to skeletal muscle regeneration. PMID: 23761625
  35. Noncanonical MMP-1-PAR1 signaling resulted in the opposite effect and led to a dedifferentiated phenotype via a different G protein pathway. MMP-1-PAR1 significantly stimulated hyperplasia and migration of SMCs. PMID: 23814055
  36. GPR64 expression in ES maintains an immature phenotype that is less sensitive to TRAIL-induced apoptosis and via its up-regulation of PGF and MMP1 orchestrates and promotes invasiveness and metastatic spread in Ewing sarcoma. PMID: 23338946
  37. Matrix metalloproteinase 13 (MMP13) is a direct target of osteoblast-specific transcription factor osterix (Osx) in osteoblasts. PMID: 23185634
  38. regulation of a11b1-dependent matrix remodeling, involving MMP-13 , might also occur in other types of fibroblasts and be an important regulatory mechanism for coordinated extracellular and intracellular collagen turnover in tissue homeostasis PMID: 23065814
  39. The findings of the present study reveal an important role of MMP-1 in OS primary tumor and metastasis formation to the lung, the major organ of osteosarcoma metastasis. PMID: 23195950
  40. microRNA miR-199a-5p down-regulation switches on wound angiogenesis by derepressing the v-ets erythroblastosis virus E26 oncogene homolog 1-matrix metalloproteinase-1 pathway PMID: 23060436
  41. Suture compression elevated the MMP-1 and 13 expressions, activated osteoclasts, reduced bone density, and induced bone resorption adjacent to the suture. It suggests that suture compression can be used for bone volume reduction PMID: 22819631
  42. These results provide evidence for an important role for MMP-13 in wound healing by coordinating cellular activities important in the growth and maturation of granulation tissue. PMID: 22880047
  43. Osteocyte perilacunar remodeling of mid-cortical bone matrix requires MMP-13 and is essential for the maintenance of bone quality. PMID: 22549931
  44. Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification PMID: 22869368
  45. demonstrate that beta-adrenergic receptor stimulation leads to MMP-13 transactivation of protease-activated receptor 1 in both cardiac fibroblasts and cardiomyocytes PMID: 22610965
  46. MMP-1, -3 and -9 genes are upregulated by West Nile virus infection in the blood-brain barrier. PMID: 22398316
  47. findings show that C/EBPbeta and RUNX2, with MMP-13 as the target and HIF-2alpha as the inducer, control cartilage degradation PMID: 22095691
  48. certain proteases, such as Mmp1, play a pivotal role in certain proteases, such as MT1-MMP, play a pivotal role in PO-induced matrix remodeling and fibrosis-induced matrix remodeling and fibrosis PMID: 22287584
  49. a novel role for ELF3 as a procatabolic factor that may contribute to cartilage remodeling and degradation by regulating MMP13 gene transcription. PMID: 22158614
  50. Wnt5a-Ror2 signaling might also be required for expression of MMP-13 gene during the development of the cartilaginous tissue. PMID: 22128168

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Subcellular Location Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families Peptidase M10A family
Tissue Specificity Detected in epidermal cells and stromal fibroblasts in wounded skin, but not in normal skin (at protein level). Detected in embryonic hypertrophic chondrocytes and newly recruited bone cells at primary ossification centers. After adult bone fracture, dete
Database Links

KEGG: mmu:17386

STRING: 10090.ENSMUSP00000015394

UniGene: Mm.5022

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