Mouse mucin-5 subtype B,MUC5B ELISA Kit

Code CSB-E15067m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

The mouse MUC5B Elisa kit is suitable for the quantitative measurement of mouse MUC5B in serum, plasma, cell culture supernates, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of MUC5B in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

MUC5B is the major gel-forming mucin in the lung that is secreted from proximal submucosal glands and distal airway secretory cells. It plays a key role in mucociliary clearance (MCC) and host defense. MUC5B has a critical protective function in the normal lung, salivary glands, esophagus, and gallbladder, and has been reported to be abnormally expressed in breast cancer. Previous studies have shown that MUC5B is important for tumor progression. Dysregulation of MUC5B expression has also been reported in airway diseases.

Target Name mucin-5 subtype B,MUC5B
Abbreviation MUC5B
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.31 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %81-94
1:2Average %96
Range %91-102
1:4Average %99
Range %93-105
1:8Average %92
Range %86-98
Recovery
The recovery of mouse MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-96
EDTA plasma (n=4)9890-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
802.236 2.141 2.189 2.098
401.879 1.801 1.840 1.749
201.461 1.369 1.415 1.324
100.853 0.793 0.823 0.732
50.512 0.496 0.504 0.413
2.50.307 0.301 0.304 0.213
1.250.195 0.186 0.191 0.100
00.093 0.088 0.091  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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