Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

Code CSB-E08676m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
myeloperoxidase-antineutrophil cytoplasmic antibody
Abbreviation
MAC Ab
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-102  
1:2 Average % 95  
Range % 83-101  
1:4 Average % 96  
Range % 90-106  
1:8 Average % 94  
Range % 82-101  
Recovery
The recovery of mouse myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA)spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 89-98  
EDTA plasma (n=4) 94 91-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.404 2.418 2.411 2.297  
20 1.956 1.904 1.930 1.816  
10 1.352 1.377 1.365 1.251  
5 0.902 0.934 0.918 0.804  
2.5 0.526 0.534 0.530 0.416  
1.25 0.311 0.326 0.319 0.205  
0.625 0.265 0.271 0.268 0.154  
0 0.112 0.115 0.114    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse MAC Ab ELISA Kit was designed for the quantitative measurement of Mouse MAC Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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