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Mouse neurotensin (NTS) ELISA kit

Mouse neurotensin (NTS) ELISA kit

Protocol MSDS

Code	CSB-EL016136MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

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Target Background

Product Details

Target Name

neurotensin

Alternative Names

Nts ELISA Kit; Neurotensin/neuromedin N [Cleaved into: Large neuromedin N ELISA Kit; NmN-125); Neuromedin N ELISA Kit; NN ELISA Kit; NmN); Neurotensin ELISA Kit; NT); Tail peptide] ELISA Kit

Abbreviation

NTS

Uniprot No.

Q9D3P9

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NTS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-89
1:2	Average %	98
	Range %	94-105
1:4	Average %	90
	Range %	85-95
1:8	Average %	107
	Range %	103-110

Recovery

The recovery of mouse NTS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	97	94-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.890	2.768	2.829	2.637
200	2.346	2.204	2.275	2.083
100	1.626	1.702	1.664	1.472
50	1.047	1.100	1.074	0.882
25	0.733	0.741	0.737	0.545
12.5	0.517	0.502	0.510	0.318
6.25	0.371	0.382	0.377	0.185
0	0.191	0.193	0.192

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse NTS ELISA Kit was designed for the quantitative measurement of Mouse NTS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

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Target Background

Function
(From Uniprot)

Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.

Gene References into Functions

Study reveals multiple populations of neurotensin neurons that provide direct afferents to the ventral tegmental area and which may regulate specific aspects of motivated behavior. PMID: 29478718
The weight loss behaviors are mediated by distinct signaling pathways: Nts action via NtsR1 is essential for the anorectic effect of the lateral hypothalamic Nts circuit, but not for regulation of locomotor or drinking behavior. PMID: 29241540
Nts-leptin receptor neurons are important neuronal hubs within the lateral hypothalamic area for hormone-mediated control of ingestive and locomotor behaviors. PMID: 28323938
findings directly link NT with increased fat absorption and obesity and suggest that NT may provide a prognostic marker of future obesity and a potential target for prevention and treatment PMID: 27193687
Data (including data from studies in transgenic mice) suggest Nts, glucagon-like peptide I, and peptide YY are closely co-expressed/co-secreted within enteroendocrine cells in ileum mucosa; however, Nts is stored in distinct/separate secretory granules. PMID: 26469136
The roles of neurotensin and its receptor NTR-1 on sperm capacitation and acrosome reaction in mice are reported. PMID: 25031361
These observations suggest the importance of neurotensin in maintaining activity of orexin neurons. PMID: 23620827
a lineage of mature enteroendocrine cells have the ability to coexpress members of a group of functionally related peptides: CCK, secretin, GIP, GLP-1, PYY, and neurotensin PMID: 23064014
Neurotensin knock-out mice do not differ from their wild-type or heterozygous littermates in either baseline or acute cocaine-stimulated locomotor activity. PMID: 21720755
Neurotensin increases EGF expression in skin dendritic cells. PMID: 21767580
NT acutely regulates D2 autoreceptor function and DA neuron excitability through PKC-mediated phosphorylation of the D2R, leading to heterologous receptor desensitization. PMID: 21233215
although neurotensin neurons in the anteroventral periventricular nucleus are targets for regulation by E(2), neurotensin does not appear to play a direct role in generating the GnRH/LH surge in the mouse PMID: 19861584
neurotensin has a role in chronic mitogen-activated protein kinase activation and cancer progression PMID: 14699144
Data show that although brain neuromedin N mRNA distribution largely overlaps in mice and rats, species differences exist in specific brain areas, and the distribution in mice resembles that of primates more than that of rats. PMID: 15193419
NT and NTR1 are part of network activated after mucosal injuries, and NT stimulates epithelial restitution, at least in part, through a COX-2 dependent pathway. PMID: 15764810
Neurotensin may have a direct role in the neuroendocrine control of feeding and energy homeostasis. PMID: 16221860
co-localization of peptides and enzyme in GHRH-eGFP neurons displays a sexual dimorphism at 3-month of age for NT, and at 8-month for tyrosine hydroxylase, while the total number of GHRH-eGFP neurons does not exhibit gender difference at either age. PMID: 17137749
[(99m)Tc]Demotensin 6 shows a favourable preclinical profile and further testing in patients is warranted to monitor its eventual applicability as a radiotracer in the diagnostic imaging of NTS1-R-positive tumours. PMID: 17594090
These results clearly indicate for the first time that NT is able to protect endocrine beta cells from external cytotoxic agents, a role well correlated with its release in the circulation after a meal. PMID: 18456542
NT-mediated IL-6 expression is nuclear factor-kappaB and PKCdelta dependent PMID: 19443690

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Subcellular Location

Secreted. Cytoplasmic vesicle, secretory vesicle.

Protein Families

Neurotensin family

Database Links

KEGG: mmu:67405

STRING: 10090.ENSMUSP00000020040

UniGene: Mm.64201

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Mouse neurotensin (NTS) ELISA kit

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Target Background