Mouse osteonectin,ON ELISA Kit

Instructions
Code CSB-E08762m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Alternative Names Sparc ELISA Kit; SPARC ELISA Kit; Basement-membrane protein 40 ELISA Kit; BM-40 ELISA Kit; Osteonectin ELISA Kit; ON ELISA Kit; Secreted protein acidic and rich in cysteine ELISA Kit
Abbreviation SPARC
Uniprot No. P07214
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 86  
Range % 83-89  
1:200 Average % 87  
Range % 82-95  
1:400 Average % 96  
Range % 94-98  
1:800 Average % 97  
Range % 91-103  
Recovery
The recovery of mouse ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 95-104  
EDTA plasma (n=4) 91 87-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.477 2.479 2.478 2.342  
1000 2.147 2.179 2.163 2.027  
500 1.694 1.672 1.683 1.547  
250 1.002 1.056 1.029 0.893  
125 0.633 0.659 0.646 0.510  
62.5 0.328 0.339 0.334 0.198  
31.25 0.224 0.214 0.219 0.083  
0 0.135 0.137 0.136    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Gene References into Functions
  1. It is a matricellular glycol that regulates cell proliferation, tissue repair, and tumorigenesis and a potential therapeutic target for conditions involving intestinal inflammation. PMID: 28582593
  2. Sparc levels in tendons are critical for proper collagen fibril maturation. PMID: 27586416
  3. miR-29 directly targeted SPARC, resulting in degradation of SPARC-encoding mRNA and reduction in the SPARC protein level. PMID: 29462611
  4. SPARC plays a crucial role in the proliferation and differentiation of C2C12 and may be involved in the link between the ECM remodeling and mitochondrial func PMID: 29420632
  5. SPARC is regulating the interplay between myeloid-derived suppressor cells and the extracellular matrix to drive the induction of epithelial-to-mesenchymal transition in tumor cells. PMID: 27681434
  6. The results demonstrate for the first time a functional role of the N-propeptide in regulating collagen fiber assembly and cell behavior and suggest that SPARC and the N-propeptide of collagen I have distinct activities in regulating collagen fiber assembly and fibroblast function. PMID: 28245286
  7. SPARC plays a key role in influencing the spatial organization of the anterior segment, potentially via modulation of collagen properties, while Hevin is not likely to be involved. PMID: 28122087
  8. An ADAMTS1 blocking antibody suppressed the SPARC-induced collagen I secretion, indicating that SPARC promoted collagen production directly through ADAMTS1 interaction. In conclusion, ADAMTS1 is an important mediator of SPARC-regulated cardiac aging. PMID: 27143554
  9. SPARC appears to be an important modulator of the actin cytoskeleton, implicating maintenance of muscular function PMID: 27908613
  10. resistivity measurements were taken on 22 mice, 11 wild-type and 11 sparc-/- (knock out for the protein SPARC: secreted protein acidic and rich in cysteine), bearing mammary carcinomas. PMID: 27099155
  11. SPARC isoforms, acting on Adipose stromal cells through distinct mechanisms, have an additive effect in inducing ASC migration. PMID: 26381424
  12. SPARC levels were not associated with efficacy in patients with MPC. This exploratory analysis does not support making treatment decisions regarding nab-paclitaxel plus gemcitabine or gemcitabine alone in MPC based on SPARC expression. PMID: 26169969
  13. SPARC is proposed to act as a critical regulator of transglutaminase activity on collagen I with implications for mechanical strength of tissues. PMID: 25827352
  14. Tumor-produced SPARC and VCAM1 are regulators of cancer extravasation. PMID: 25925867
  15. The results of this study indicated that the loss of p53 by deletion/mutation in the early stages of glioma formation may cooperate with the induction of SPARC to potentiate cancer cell survival and escape from immune surveillance. PMID: 24862407
  16. This work suggests that SPARC could affect anxiety-related behavior by modulating neuronal activity, and that depression-related behavior is dependent upon the adult expression of SPARC, which affects adult brain function. PMID: 22950524
  17. SPARC has a significant role in the development of concanavalin A -induced severe liver injury. PMID: 25410742
  18. SPARC acts as an important mediator of age-related cardiac inflammation by increasing the expression of macrophage M1 markers and decreasing M2 markers. PMID: 25877699
  19. SPARC inhibits growth factor-induced signaling in both INS-1 beta cells and primary mouse islets, and inhibits IGF-1-induced proliferation of INS-1 beta cells. PMID: 25204658
  20. Age and SPARC change the extracellular matrix composition of the left ventricle. PMID: 24783223
  21. findings suggest that SPARC plays a crucial role in the regulation of early B lymphopoiesis. PMID: 24598056
  22. SPARC-deficient mice display higher resistance to serial treatment with the chemotherapeutic agent 5-fluorouracil (5-FU). PMID: 24833352
  23. effects of age and the expression of SPARC on extracellular matrix production by cardiac fibroblasts PMID: 24223185
  24. SPARC KO mice had less inflammation. PMID: 24204877
  25. These results demonstrate that there is a delicate temporal regulation of SPARC and that disturbances in this may have an adverse effect on muscle regeneration. PMID: 23670848
  26. These findings suggest that SPARC plays a critical role in the maintenance of miMEFs without loss of cell number and might be a key component for supporting the culture of human PSCs. PMID: 23661086
  27. Loss of SPARC not only upregulates atrogin 1 expression but also enhances transforming growth factor (TGF)beta signaling, which may in turn cause muscle atrophy. PMID: 23424163
  28. data suggest that SPARC plays a significant role in liver fibrogenesis. Interventions to inhibit SPARC expression are suggested as promising approaches for liver fibrosis treatment PMID: 23408952
  29. new insight into the biological functions of SPARC PMID: 23349702
  30. These findings suggest that exercise stimulates SPARC secretion from muscle tissues and that SPARC inhibits colon tumorigenesis by increasing apoptosis. PMID: 22851666
  31. SPARC modulates intraocular pressure regulation in the eye, and also correlates with trabecular meshwork morphology. PMID: 23422826
  32. Using a chemical carcinogenesis model in Sparc-deficient mice and their wild-type littermates, we found that loss of SPARC accelerated the development of urothelial preneoplasia, neoplasia, and metastasis and was associated with decreased survival. PMID: 23321672
  33. This sdtudy demonistrated that SPARC regulates microgliosis and functional recovery following cortical ischemia and brain injury. PMID: 23467362
  34. SPARC and SC1 may share significant homology but are likely to have distinct but complementary roles in central nervous system development. PMID: 22173850
  35. SPARC-null mice develop region-specific, age-dependent hypersensitivity to cold, icilin, and capsaicin; axial discomfort; motor impairment; and reduced physical function. PMID: 22414871
  36. SPARC suppresses angiogenesis of gastric cancer by down-regulating the expression of VEGF and MMP-7 PMID: 22957090
  37. Stromal SPARC contributes to the detrimental fibrotic changes associated with myeloproliferation whereas its deficiency favors myeloid cell expansion. PMID: 22955913
  38. SPARC promotes the development of erythroid progenitors, but does not affect terminal erythroid differentiation. PMID: 22687753
  39. The Sparc(-/-) mice had reduced survival and fewer well-defined abdominal lesions compared with WT controls after IP injection, whereas no differences were observed in survival or abdominal lesions between Sparc(-/-) and WT mice after OT injection. PMID: 22003835
  40. Osteonectin may suppress prostate cancer pathogenesis. PMID: 22525512
  41. aberrant TGFbeta1-activation in the absence of host SPARC contributes significantly to tumor progression and SPARC, by controlling ECM deposition and maturation, can regulate TGFbeta availability and activation PMID: 22348081
  42. SPARC influences the shuttling of Pax6 via the TGF-beta/Smad signaling pathway. PMID: 22539874
  43. follicular dendritic cell networking toward Th17 responses PMID: 21962567
  44. Data indicate that intracellular SPARC-positive vesicles did not undergo visible degradation. PMID: 21949685
  45. SPARC and connective tissue growth factor appeared to be involved in the same biological pathway PMID: 21978691
  46. DNA methylation down-regulates SPARC expression in chronic low back pain (LBP) in pre-clinical models and in patients with chronic LBP. PMID: 21867537
  47. SPARC regulates collagen in the heart by modulating procollagen processing and interactions with fibroblast cell surfaces. PMID: 21666116
  48. These results identify hevin as a positive and SPARC as a negative regulator of synapse formation. PMID: 21788491
  49. SPARC deletion preserves left ventricular function at day 3 post-myocardial infarction but may be detrimental for the long-term response due to impaired fibroblast activation. PMID: 21602472
  50. SPARC promotes pericyte migration by diminishing TGF-beta activity and identify a novel function for endoglin in controlling pericyte behavior. PMID: 21708981

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Protein Families SPARC family
Database Links

KEGG: mmu:20692

STRING: 10090.ENSMUSP00000018737

UniGene: Mm.291442

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