Mouse ovalbumin specific IgE,OVA sIgE ELISA Kit

This ELISA kit provides quantitative measurement of ovalbumin-specific IgE (OVA sIgE) in mouse serum and plasma, supporting research in allergic sensitization and Th2-driven immune responses. Because OVA sIgE levels vary dramatically between baseline and allergen-challenged states in murine asthma and food allergy models, the kit is designed to capture this dynamic range while detecting the low concentrations present during early sensitization phases.

Detection Range: The 0.24–15 ng/mL range spans the concentrations typically observed from initial OVA sensitization through peak allergic challenge responses in commonly used BALB/c and C57BL/6 models, minimizing the need for extensive serial dilutions while covering both moderate and robust responders.

Sensitivity: At 0.12 ng/mL, the kit detects OVA sIgE in mice during early sensitization timepoints or in low-responder strains where antigen-specific IgE remains minimal before booster immunizations.

Sample Compatibility: Validation in both serum and plasma allows researchers to use matched samples across longitudinal bleeding schedules and terminal collections, accommodating repeated submandibular bleeds alongside cardiac puncture endpoints within the same study.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate the limited blood volumes obtainable from serial retro-orbital or submandibular sampling in mice, preserving samples for parallel measurement of total IgE or cytokines.

This kit is particularly suited for OVA-induced airway inflammation models, murine food allergy studies, and evaluation of immunotherapeutic interventions targeting Th2 sensitization.