Mouse pancreatic alpha amylase (AMY2A)ELISA kit

Code CSB-EL001689MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name pancreatic alpha amylase (AMY2A)
Alternative Names Amy2 ELISA Kit; Amy2a ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
Abbreviation AMY2A
Uniprot No. P00688
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 9.38 mU/mL-600 mU/mL
Sensitivity 2.3 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 92-105
1:2 Average % 92
Range % 88-96
1:4 Average % 86
Range % 80-92
1:8 Average % 89
Range % 84-97
The recovery of mouse AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-97
EDTA plasma (n=4) 101 94-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
600 2.680 2.714 2.697 2.563
300 2.164 2.235 2.200 2.066
150 1.467 1.421 1.444 1.310
75 0.759 0.811 0.785 0.651
37.5 0.533 0.564 0.549 0.415
18.75 0.317 0.326 0.322 0.188
9.38 0.227 0.241 0.234 0.100
0 0.128 0.139 0.134  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Subcellular Location Secreted, extracellular space.
Protein Families Glycosyl hydrolase 13 family
Database Links

KEGG: mmu:100043684

STRING: 10090.ENSMUSP00000137025

UniGene: Mm.275426

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