Mouse ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) (RNASE2) ELISA kit

Instructions
Code CSB-E17924m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)
Alternative Names Rnase2 ELISA Kit; Ear4 ELISA Kit; Non-secretory ribonuclease ELISA Kit; EC 4.6.1.18 ELISA Kit; Eosinophil cationic-type ribonuclease 4 ELISA Kit; MR-4 ELISA Kit; Ribonuclease 2 ELISA Kit; RNase 2 ELISA Kit
Abbreviation RNASE2
Uniprot No. O35291
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse RNASE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-93
1:2 Average % 100
Range % 97-104
1:4 Average % 86
Range % 83-89
1:8 Average % 99
Range % 95-104
Recovery
The recovery of mouse RNASE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-92
EDTA plasma (n=4) 108 104-112
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.441 2.501 2.471 2.358
100 2.020 1.968 1.994 1.881
50 1.301 1.324 1.313 1.200
25 0.725 0.705 0.715 0.602
12.5 0.407 0.412 0.410 0.297
6.25 0.304 0.311 0.308 0.195
3.12 0.195 0.199 0.197 0.084
0 0.112 0.114 0.113  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.
Subcellular Location Lysosome, Cytoplasmic granule
Protein Families Pancreatic ribonuclease family
Database Links

KEGG: mmu:53877

UniGene: Mm.377124

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