Mouse sex hormone-binding globulin,SHBG ELISA Kit

Code CSB-E08233m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
sex hormone-binding globulin
Alternative Names
Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
1.3 ng/mL-50 ng/mL
0.33 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-97  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 85  
Range % 81-90  
1:8 Average % 89  
Range % 83-95  
The recovery of mouse SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-95  
EDTA plasma (n=4) 109 104-114  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.344 2.491 2.418 2.370  
27 1.768 1.822 1.795 1.747  
13 1.003 1.042 1.023 0.975  
3.3 0.376 0.365 0.371 0.323  
1.3 0.175 0.189 0.182 0.134  
0 0.046 0.049 0.048    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse SHBG ELISA Kit was designed for the quantitative measurement of Mouse SHBG protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 1.3 ng/mL-50 ng/mL and the sensitivity is 0.33 ng/mL.

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Target Background

(From Uniprot)
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Gene References into Functions
  1. SHBG suppresses inflammation and lipid accumulation in macrophages and adipocytes, which might be among the mechanisms underlying the protective effect of SHBG, that is, its actions which reduce the incidence of metabolic syndrome PMID: 30046278
  2. A wave of apoptosis during the pre-pubertal period is required for normal spermatogenesis to develop, and our data indicate that this apoptotic wave may be regulated by ABP and/or androgens. PMID: 12831397
  3. USF transcription factors repress SHBG transcription in Sertoli cells through an interaction with FP4 within its proximal promoter PMID: 15574421
  4. monosaccharide-induced lipogenesis reduced hepatic HNF-4alpha levels, which in turn attenuated SHBG expression. PMID: 17992261
Subcellular Location
Database Links
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