Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit

Code CSB-E11155m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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Product Details

Target Name
myosin, heavy chain 1, skeletal muscle, adult
Alternative Names
Myh1 ELISA Kit; Myosin-1 ELISA Kit; Myosin heavy chain 1 ELISA Kit; Myosin heavy chain 2x ELISA Kit; MyHC-2x ELISA Kit; Myosin heavy chain ELISA Kit; skeletal muscle ELISA Kit; adult 1 ELISA Kit
Abbreviation
MYH1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays):CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse sMHC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 85-99    
1:2 Average % 102    
Range % 97-106    
1:4 Average % 85    
Range % 81-90    
1:8 Average % 104    
Range % 100-108    
Recovery
The recovery of mouse sMHC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 85-96    
EDTA plasma (n=4) 87 92-93    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1000 2.009 2.061 2.035 1.862    
500 1.424 1.402 1.413 1.240    
250 0.980 0.951 0.966 0.793    
125 0.577 0.589 0.583 0.410    
62.5 0.380 0.398 0.389 0.216    
31.2 0.271 0.268 0.270 0.097    
15.6 0.223 0.215 0.219 0.046    
0 0.176 0.170 0.173      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse MYH1 ELISA Kit was designed for the quantitative measurement of Mouse MYH1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Muscle contraction.
Gene References into Functions
  1. Data suggest that single muscle immobilization induces a shift of myosin heavy chain (MHC) isoforms composition toward a faster contractile phenotype and decreases the polymorphic profile of single fibres, and that activation of p38 and JNK could be a potential mechanism involved in these contractile phenotype modifications during muscle immobilization. PMID: 27383612
  2. p38alpha/beta MAPKs-mediated coactivator recruitment at a proximal MEF-2 site is important for MyHCIId/x gene regulation in skeletal muscle. PMID: 17210568
  3. The maintenance of the relative expression of slow MHC-1 protein is independent of neuromuscular activity in mice. PMID: 18945940
Subcellular Location
Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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