Mouse soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

Instructions
Code CSB-E08135m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name platelet/endothelial cell adhesion molecule
Alternative Names Pecam1 ELISA Kit; Pecam ELISA Kit; Pecam-1 ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit
Abbreviation PECAM1
Uniprot No. Q08481
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 87
Range % 82-91
1:200 Average % 96
Range % 92-100
1:400 Average % 104
Range % 100-110
1:800 Average % 90
Range % 85-94
Recovery
The recovery of mouse sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 88-96
EDTA plasma (n=4) 84 80-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.923 2.814 2.869 2.771
100 2.059 2.148 2.104 2.006
50 1.239 1.159 1.199 1.101
25 0.687 0.697 0.692 0.594
12.5 0.419 0.405 0.412 0.314
6.25 0.281 0.273 0.277 0.179
3.12 0.184 0.186 0.185 0.087
0 0.095 0.101 0.098  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis
Gene References into Functions
  1. Study confirmes that the presence of functional PECAM1 on the endothelium promotes a proliferative tumor cell phenotype in vivo and in vitro. These proproliferative effects were mediated by soluble endothelialderived factors that are dependent on PECAM1 homophilic ligand interactions, but are independent of PECAM1dependent signaling. PMID: 29845213
  2. PECAM is required for fibronectin assembly in vascular endothelium during flow-mediated vascular remodeling. PMID: 28120882
  3. Differences in trafficking of CD31(+) cytotoxic T lymphocytes during acute influenza infection could modulate tolerance and contribute to a dampened adaptive immune response in neonates PMID: 28355204
  4. Stromal DNA replication was highly activated in the uterus of CD31(-/-) mice, manifested by upregulated cyclin series signaling and PCNA expression after E2 + P4 treatment. Collectively, CD31 inhibits E2 -mediated epithelial proliferation via recruitment and phosphorylation of SHP-2 upon receiving P4 signal in early pregnancy. PMID: 28780571
  5. findings suggest that PECAM-1 enhances SDF-1-induced chemotaxis by augmenting and prolonging activation of the PI3K/Akt/mTORC1 pathway and Rap1 and that PECAM-1, at least partly, exerts its activity by inhibiting SDF-1-induced internalization of CXCR4 PMID: 28974577
  6. The data in this study provide evidence for the differential involvement of PECAM-1-ligand interactions in PECAM-1-dependent motility and the extension of filopodia. PMID: 27895229
  7. RrgA, binds both polymeric immunoglobulin receptor (pIgR) and PECAM-1, whereas the choline binding protein PspC binds, but to a lower extent, only pIgR PMID: 28515075
  8. Both the number of lung CD31-CD45-Sca-1+ cells and the expression levels of the Shh signaling pathway were downregulated in the lung tissues of mice with pulmonary emphysema. These cells and Shh signaling pathway are reactivated during acute adenovirus infection. PMID: 28352167
  9. In FVB/n mice, PECAM functions upstream of CD99 in leukocyte diapedesis and blocking antibodies against either molecule arrest neutrophils before they traverse the endothelium. However, in C57BL/6 mice, PECAM and CD99 appear to function at a different step, as the same antibodies arrest leukocyte migration through the endothelial basement membrane. PMID: 27422987
  10. These studies indicate a role for PECAM-1 in enhancing the inhibitory functions of TGF-beta in T cells PMID: 26956486
  11. PECAM1 acts upstream of Gab1, promoting Gab1/Akt/eNOS phosphorylation and activation, thereby confers EC responsiveness to flow. PMID: 26706435
  12. Radiation-induced stress conditions induce a transient accumulation of granulocytes within the liver by down-regulation/absence of PECAM-1. PMID: 26177067
  13. CD31 gene transfer is sufficient to recapitulate the cytoprotective mechanisms in CD31(-) pancreatic beta cells, which become resistant to immune-mediated rejection when grafted in fully allogeneic recipients PMID: 26392551
  14. PECAM1+ melanoma cells form vascular channels PMID: 25335460
  15. Nck promoted oxidative stress-induced activation of NF-kappaB by coupling the tyrosine phosphorylation of PECAM-1 (platelet endothelial cell adhesion molecule-1) to the activation of p21-activated kinase PMID: 25714462
  16. PECAM-1 has a role in mediating the profibrotic and prometastasic environment caused by ethanol in endothelial cells PMID: 24734240
  17. the T cell co-inhibitory receptor CD31 prevents the formation of functional T/B immunological synapses and that therapeutic strategies aimed at sustaining CD31 signaling will attenuate the development of autoimmune responses in vivo. PMID: 25277651
  18. We have identified a single locus, at 35.8 Mb on murine chromosome 2, associated with PECAM-independent inflammation in the thioglycollate peritonitis (TGP) model. We suggest a name of Pitgp for this locus. PMID: 19180231
  19. PECAM is a sialic acid binding lectin and that this binding property supports endothelial cell survival. PMID: 25135639
  20. Cross-linking PECAM-1 on the surface of wild-type mouse platelets with antibodies against the 6th Ig domain decreased GPIbalpha on the platelet surface. No such decrease was seen in PECAM-1-deficient mouse platelets. PMID: 24969778
  21. catalytically active NEU1 inhibits in vitro angiogenesis through desialylation of its substrate, CD31 PMID: 24550400
  22. CD44 regulation of endothelial cell proliferation and apoptosis via modulation of CD31 and VE-cadherin expression. PMID: 24425872
  23. PECAM-1 (and caveolae) are parts of the mechanosensing machinery that generates superoxide with loss of shear. PMID: 24077950
  24. The most differentially expressed target in mantle cell lymphoma was CD31/PECAM1 and VEGF positively regulated in vivo growth. PMID: 23537525
  25. PECAM-1 has a regulatory role in venous thrombus resolution. PMID: 24081660
  26. The potential of micron-sized particles of iron oxide (MPIO) conjugated with PECAM-1-targeted antibodies, that is, alphaPECAM-1-MPIO, to expose stroke-induced PECAM-1 upregulation with molecular MRI was assessed. PMID: 23740809
  27. Data indicate a fundamental role of PECAM-1 in the inhibitory effects of statins on platelet activation. PMID: 24030383
  28. These results suggest that PECAM-1 may have multiple functions in resistance to infection with S. Typhimurium. PMID: 23509149
  29. PVR resides in the recently identified lateral border recycling compartment, similar to PECAM and CD99. PMID: 23333754
  30. PECAM1(+)/Sca1(+)/CD38(+) vascular cells could proliferate and differentiate into myofibroblast-like cells in wound repair. PMID: 23308177
  31. PECAM-1 on bone marrow cells is proatherogenic irrespective of the hemodynamic environment, however endothelial cell PECAM-1 is antiatherogenic in high shear environments. PMID: 23372062
  32. T cell-endothelial cell interactions mediated by CD31 molecules are required for efficient localization of naive T lymphocytes to secondary lymphoid tissue and constitutive recirculation of primed T cells to nonlymphoid tissues. PMID: 22966083
  33. We propose that CD31-mediated modulation of memory T cell chemokinesis is a key mechanism by which this molecule contributes to the homeostatic regulation of effector T cell immunity. PMID: 22724015
  34. CD31 peptides could represent a new class of drugs intended to prevent the inflammatory cell processes underlying progression of atherosclerosis and abdominal aortic aneurysm development. PMID: 22293851
  35. Report role of mechanotransducer CD31 in modulation of functional responses of endothelial cells linked to angiogenesis and inflammation by shear stress. PMID: 21898411
  36. PECAM-1 dampens cytokine levels during LPS-induced endotoxemia by regulating leukocyte trafficking. PMID: 22119535
  37. Postulate that SCA1(+)/CD31(+) cardiac side population cells may represent endothelial progenitor cells in the mouse heart. PMID: 21615679
  38. a novel role for CD31 in regulating CD4 T cell homeostasis. PMID: 21734076
  39. Immature mast cells can undergo a tightly regulated extravasation cascade consisting of rolling on and adhesion to endothelium and followed by directed diapedesis and reveal E-selectin, P-selectin, VCAM-1 and PECAM-1 as required adhesion molecules. PMID: 20507363
  40. PECAM-1 expression modulates proangiogenic properties of endothelial cells, and these activities are significantly influenced by alternative splicing of its cytoplasmic domain. PMID: 20810911
  41. In an ischemia model, PECAM-1 plays a novel role in arteriogenesis and collateral-dependent blood flow as the first molecule that determines preexisting collateral diameter. PMID: 20930147
  42. CD31 functions as a nonredundant comodulator of T-cell responses, which specializes in sizing the ensuing immune response by setting the threshold for T-cell activation and tolerance, while preventing memory T-cell death PMID: 20978210
  43. PECAM-1-KO and chimeric mice revealed that its metastasis-promoting effects are mediated specifically through vascular endothelial cell (VEC) PECAM-1. PMID: 20926749
  44. Bone marrow-CD31(+) cells represent highly angiogenic and vasculogenic cells and can be a novel and highly promising source of cells for cell therapy to treat ischemic cardiovascular diseases. PMID: 20634489
  45. Ligustrazine could enhance the CD31 expression on the surface of bone marrow nuclear cells after syngenic BMT in mice. PMID: 16463659
  46. absence of alpha2,6-sialic acid down-regulated the tyrosine phosphorylation of PECAM and recruitment of Src homology 2 domain-containing protein-tyrosine phosphatase 2 and rendered the cells more prone to mitochondrion-dependent apoptosis PMID: 20048157
  47. plays an important role in the progression of collagen-induced arthritis and an inhibitor might have therapeutic value for clinical treatment of rheumatoid arthritis PMID: 11949889
  48. PECAM-1 acts as a counterregulator of immediate hypersensitivity reactions and allergic disease susceptibility and severity, and a negative modulator of antigen-dependent IgE-mediated mast cell activation. PMID: 12055265
  49. acts as a regulator of B-cell development, B-cell antigen receptor (BCR)-mediated activation, and autoimmune disease. PMID: 12070026
  50. alternatively spliced PECAM-1 isoforms in hematopoietic cells and platelets may have roles in hematopoiesis and transendothelial migration PMID: 12397602

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Membrane raft, Cell junction
Tissue Specificity Isoform 1 and isoform 3 are expressed in lung and platelets.
Database Links

KEGG: mmu:18613

STRING: 10090.ENSMUSP00000079664

UniGene: Mm.343951

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