Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

Code CSB-E08710m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation TCC C5b9
Target Name terminal complement complex C5b-9,TCC C5b-9
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %88
Range %84-96
1:200Average %98
Range %91-105
1:400Average %95
Range %91-99
1:800Average %92
Range %88-96
Recovery
The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-97
EDTA plasma (n=4)9792-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.684 2.810 2.747 2.596
252.054 2.212 2.133 1.982
12.51.426 1.446 1.436 1.285
6.250.854 0.867 0.861 0.710
3.120.622 0.585 0.604 0.453
1.560.337 0.350 0.344 0.193
0.780.258 0.264 0.261 0.110
00.148 0.154 0.151  
References
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Q&A and Customer Reviews

Q&A
Q:

I would like to know how you make mouse TCC for this kit, or what is the source of mouse TCC in this kit.

A:
Thanks for your inquiry. C5 TCC C5b-9 is the product produced from common end effect by complement activation pathway.C5 can decompose into C5a and C5b.C5b combines with C6,C7,C8 ,C9 and forms to be C5b-9.For the specific information,pls refer to https://en.wikipedia.org/wiki/Classical_complement_pathway
#CSB-E08710m,Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit,this kit is for specific to detection on mouse TCC C5b-9.This kit is kind of special,with the standard prepared with special method.But the specific information can not be disclosed.Hope you could understand.
Q:

For the standard protein, please let us check whether the gene introduced into the HEK293 cells by viral infection method or not.

A:
Thanks for your inquiry. Our elisa kits do not contain any components expressed by virus.And the manufacturing operations are seriously standard and professional .There is no viral pollution.
Customer Reviews

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