Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

Name: Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08710m

Citations (4) Protocol MSDS

Code	CSB-E08710m
Size	96T,5×96T,10×96T
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Product Details
Citations (4)
Related Products
Customer Reviews and Q&A

Target Name

terminal complement complex C5b-9,TCC C5b-9

Abbreviation

TCC C5b9

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-96
1:200	Average %	98
	Range %	91-105
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

Recovery

The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.684	2.810	2.747	2.596
25	2.054	2.212	2.133	1.982
12.5	1.426	1.446	1.436	1.285
6.25	0.854	0.867	0.861	0.710
3.12	0.622	0.585	0.604	0.453
1.56	0.337	0.350	0.344	0.193
0.78	0.258	0.264	0.261	0.110
0	0.148	0.154	0.151

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse TCC C5b9 ELISA Kit was designed for the quantitative measurement of Mouse TCC C5b9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .

The Role of von Willebrand Factor in the Pathogenesis of C3 Glomerulopathy Z Hua, Y Chen, S Han, X Yu, Y Tan, F Yu,Kidney International Reports,2025
Inflammation resolution-based treatment of atherosclerosis using biomimetic nanocarriers loaded with specialized pro-resolving lipid mediators M Anghelache, G Voicu, R Anton, F Safciuc,Materials Today Bio,2025
Plasma protein adsorption on Fe3O4-PEG nanoparticles activates the complement system and induces an inflammatory response Escamilla-Rivera V, et al,International Journal of Nanomedicine,2019
In vitro and in vivo models of renal ischemia reperfusion injury Zwaini.et al,University of Leicester,2017

■ Customer Reviews

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■ Q&A

I would like to know how you make mouse TCC for this kit, or what is the source of mouse TCC in this kit.

Thanks for your inquiry. C5 TCC C5b-9 is the product produced from common end effect by complement activation pathway.C5 can decompose into C5a and C5b.C5b combines with C6,C7,C8 ,C9 and forms to be C5b-9.For the specific information,pls refer to https://en.wikipedia.org/wiki/Classical_complement_pathway
#CSB-E08710m,Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit,this kit is for specific to detection on mouse TCC C5b-9.This kit is kind of special,with the standard prepared with special method.But the specific information can not be disclosed.Hope you could understand.

For the standard protein, please let us check whether the gene introduced into the HEK293 cells by viral infection method or not.

Thanks for your inquiry. Our elisa kits do not contain any components expressed by virus.And the manufacturing operations are seriously standard and professional .There is no viral pollution.

Does your kit measure the soluble C5b-C9 in the mouse?

This kit can detect soluble TCC C5b-9. This kind of writing "C5b-C9" is generally not used at present.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.